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US 2014/0349347 A1Published application
Synthesis of n-acetyl-d-neuraminic acid
AssigneeGlycom A/S
InventorsAndreas Schroven, Gyula Dekany, Ionnis Vrasidas
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Abstract
Method of making NANA from NAM, which may itself be made from fructose. The NAM is treated with pyruvic acid or a pyruvate in the presence of a NANA aldolase having at least 70% sequence similarity or homology to the amino acid sequence of SEQ. ID. NO. 1.
Claims17
A method for making NANA from NAM and that comprises a step of:
- treating NAM with pyruvic acid or a pyruvate in the presence of a NANA aldolase characterized by at least 70% sequence similarity or homology to the amino acid sequence of SEQ. ID. NO. 1.
A method of making NANA from D-fructose, comprising the steps of:
- i) making, from D-fructose and R1NH2 via Heyns-rearrangement, an N-substituted D-mannosamine derivative of formula 1
- wherein R1 is a group removable by hydrogenolysis;
- ii) hydrogenating and then acetylating the N-substituted D-mannosamine derivative of formula 1 to make NAM;
- and then
- iii) treating the NAM with pyruvic acid or a pyruvate in the presence of a NANA aldolase.
2 more elements
Dependencies are inferred from the claim text.