Use of lysozyme as a tag
AssigneeMorphosys AG
InventorsStefan Haertle, Sebastian Jaeger, Daniela Daubert
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Abstract
The present disclosure provides a method to express and purify polypeptides and proteins. In the present disclosure the use of lysozyme as a fusion partner is disclosed. Furthermore, purification methods to isolate lysozyme-tagged polypeptides and proteins via lysozyme-specific antibodies are described. More specifically, the present disclosure provides a method to express and purify monomeric polypeptides and proteins by using lysozyme as a tag.
Claims13
A method for the production of an isolated monomeric polypeptide or protein, said method comprising the steps of
- (a) expressing said monomeric polypeptide or protein as a fusion protein in a host cell, wherein said fusion protein comprises said monomeric polypeptide or protein and lysozyme and
- (b) isolating said fusion protein.
A kit comprising an expression vector encoding a fusion protein which comprises a polypeptide or protein and lysozyme and an antibody specific for lysozyme.
Dependencies are inferred from the claim text.