Production of recombinant proteins with simple glycoforms
AssigneeSigma-Aldrich Co.
InventorsNan Lin, Natalie Sealover, Henry George, Kevin Kayser
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Abstract
The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
Claims20
A genetically modified cell line deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1).
A method for producing a cell deficient in Mgat1 comprising transfecting a host cell with a targeting endonuclease that targets a specific sequence in a chromosomal sequence encoding Mgat1.
A method for producing a recombinant protein having one or more terminal mannose residues, the method comprising:
- a) introducing a nucleic acid encoding the recombinant protein into a cell line in which a targeting endonuclease inactivated all chromosomal sequences encoding Mgat1 such that the cell line produces no Mgat1;
- and
- b) expressing the recombinant protein.
Dependencies are inferred from the claim text.