Mbth-like proteins in the production of semi synthetic antibiotics
AssigneeDSM Sinochem Pharmaceuticals Netherlands B.V.
InventorsUlrike Maria Mueller, Rémon Boer, Roelof Ary Lans Bovenberg
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Abstract
The present invention relates to the preparation of β-lactam antibiotics comprising contacting 4-hydroxyphenylglycine or phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase and subsequent cyclization using an isopenicillin N synthase in the presence of an MbtH-like protein and to a host cell equipped to perform such preparation.
Claims11
A method for the preparation of an N-α-amino-4-hydroxyphenylacetyl or an N-α-am inophenylacetyl β-lactam antibiotic comprising the steps of:
- (a) contacting the amino acids 4-hydroxyphenylglycine or phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase to give a tripeptide 4-hydroxyphenylglycyl-cysteinyl-valine or a tripeptide phenylglycyl-cysteinyl-valine, respectively;
- (b) contacting the tripeptide obtained in step (a) with an isopenicillin N synthase,
- characterized in that an MbtH-like protein is present.
A eukaryotic host cell comprising a non-ribosomal peptide synthetase, an isopenicillin N synthase and a polynucleotide allowing the expression of an MbtH-like protein.
Dependencies are inferred from the claim text.