Raman spectroscopy for detection of glycated analytes
AssigneeMassachusetts Institute of Technology
InventorsRamachandra Dasari, Ishan Barman, Narahara Chari Dingari, Jeon Woong Kang
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Abstract
The present invention relates to the optical measurement of blood analytes, such as glycated hemoglobin (HbA1c) and serum albumin as a functional metric of mean blood glucose in the diagnosis of diabetic patients. Non-enhanced Raman spectroscopy is employed as the analytical method for quantitative detection of blood analytes. Using processing techniques, non-enzymatic glycosylation (glycation) of the analytes results in measurable and highly reproducible changes in the acquired spectral data, which enable the accurate measurements and classification of glycated and unglycated analytes.
Claims50
A method for detecting of one or more analytes in a sample using Raman shifted light, comprising the steps of:
- obtaining a solution of the sample comprising a glycated analyte;
- concentrating the sample;
- acquiring Raman data from the concentrated sample;
- and
- analyzing the data to determine a level of at least the glycated analyte in the sample.
A system for detecting of a glycated analyte in a sample using Raman shifted light comprising:
- a sample processing device to concentrate a sample including a glycated analyte;
- a light delivery system to illuminate the sample with light;
- a detector that detects Raman scattered light from the sample;
- and
- a data processor that processes Raman data to determine a level of at least the glycated analyte in the sample.
A method for the simultaneous detection of glycated hemoglobin and glycated serum albumin comprising the steps of:
- obtaining a solution of the sample comprising hemoglobin and serum albumin;
- depositing a portion of the solution onto a surface and drying the solution to form a dried sample;
- acquiring Raman data from the dried sample;
- and
- analyzing the Raman data to determine levels of glycated hemoglobin and serum albumin.
An imaging system comprising a Raman measurement system to measure Raman data of a sample and an imaging system to image the sample.
Dependencies are inferred from the claim text.