In vitro modelling of haematopoietic stem cell medullary nests: a tool for studying the regulation of haematopoiesis, evaluating the nesting potential of a haematopoietic graft and testing the pharmacotoxicology of medicaments

A kit comprising:

• a. a calcium biomaterial;
• b. osteoclasts or a set of cytokines and/or growth factors allowing differentiation of hematopoietic stem cells (HSC) and/or hematopoietic progenitors (HP) into osteoclasts;
• c. endothelial cells, or a set of cytokines and/or growth factors allowing differentiation of circulating endothelial progenitors or mononuclear cells of bone marrow into endothelial cells;
• d. mesenchymatous stem cells (MSCs) and/or osteoblasts and/or adipocytes and optionally a set of cytokines and/or growth factors allowing differentiation of MSCs into osteoblast/adipocytes;
• and/or osteoblasts and/or adipocytes;
• and
• e. optionally one or more component(s) of the extracellular matrix (ECM).