Methods of enhancing genetic diagnosis
AssigneeBiolamina AB
InventorsKarl Tryggvason, Kristian Tryggvason
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Abstract
The present disclosure relates to the use of laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. Methods of enhancing genetic diagnosis by expanding a single stem cell on laminin-521 prior to PCR amplification are disclosed herein.
Claims13
A method of performing pre-implantation genetic diagnosis, comprising:
- receiving a first stem cell from a multi-cell fertilized embryo;
- expanding the first stem cell into two or more descendant stem cells on a substrate comprising a laminin and a cadherin, wherein the laminin is an intact protein or a protein fragment of laminin-521 or laminin-511;
- extracting DNA from the two or more descendant stem cells;
- amplifying the extracted DNA;
- and
- analyzing the amplified DNA for a genetic disorder.
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Dependencies are inferred from the claim text.