Method and kit for protozoa characterization
OwnerStephen E. Fry, Jeremy Ellisinventors
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Abstract
Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples.
Claims26
A protozoa detection method for a reaction mixture capable of amplifying a segment of the nucleic acid target to produce an amplicon, the method comprising:
- amplifying the segment of the nucleic acid target to produce the amplicon;
- and
- determining by detecting the amplicon that a sample contains one or more protozoa or that the sample has an increased likelihood of containing the one or more protozoa if the amplicon.
A kit for detecting protozoa, the kit comprising:
- at least one protozoal forward primer;
- and
- at least one protozoal reverse primer.
A method for determining whether a sample contains or has an increased likelihood of containing one or more protozoa in a reaction mixture, wherein the reaction mixture comprises at least one forward primer, at least one reverse primer, and a nucleic acid target from the sample;
- wherein the reaction mixture is capable of amplifying a segment of the nucleic acid target to produce an amplicon;
- and wherein production of the amplicon is primed by the at least one forward primer and the at least one reverse primer, the method comprising the steps of:
- (a) incubating the vessel under conditions allowing production of the amplicon if the sample contains one or more protozoa;
- and
- (b) determining that the sample contains one or more protozoa or that the sample has an increased likelihood of containing one or more protozoa if the amplicon is detected.
Dependencies are inferred from the claim text.