Exonuclease cycling assay
AssigneeGeorgetown University
InventorsMark Danielsen, Berenice Alfonso, Bolor Tumurpurev
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Abstract
The present invention relates to a novel method for detecting a target polynucleotide having a target sequence, comprising hybridizing the target polynucleotide with a probe to form a hybrid; exposing the hybrid to a 5′ exonuclease so that the probe in the hybrid is digested and the target polynucleotide is dissociated from the digested probe; repeating the hybridization step and the digestion step; and detecting the digested probes. The presence of the digested probes indicates the presence of the target polynucleotide.
Claims20
A method for detecting a target polynucleotide having a target sequence, comprising
- (a) hybridizing the target polynucleotide with a probe having a sequence complementary with the target sequence and lacking a phosphorylated 5′ end, whereby a hybrid of the probe and the target polynucleotide is formed, wherein the hybrid comprises a hybridization region formed between the target sequence and the probe;
- (b) exposing the hybrid to a 5′ exonuclease, whereby the probe in the hybrid is digested and the target polynucleotide is dissociated from the digested probe;
- (c) repeating steps (a) and (b);
- and
- (d) detecting the digested probes;
- wherein the presence of the digested probes indicates the presence of the target polynucleotide.
Dependencies are inferred from the claim text.