Kit useful for detecting of donkey meat present in meat
OwnerHasan Yetim, Fikrettin Sahin +1inventors
InventorsHasan Yetim, Fikrettin Sahin, Zulal Kesmen
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Abstract
The present invention relates to a kit comprising specific primer-probe set which is used for detecting donkey meat present in meat products by means of real-time PCR TaqMan probe technique. In the present invention contamination of donkey meat up to 0.1 picogram is enabled to be detected. The specific detection of donkey meat is made possible in raw and heat treated meat mixtures, by means of no cross-reaction until 40th cycle with horse, pork, bovine, ovine, chicken and turkey species with the kit specific to the donkey species.
Claims18
1-6. (canceled)
A kit for detecting donkey meat in meat products comprising:
- a forward primer, a reverse primer;
- and a probe which are complementary to the region on mitochondrial NADH dehydrogenase subunit 5 (ND5) gene;
- and a primer-probe set in detection of donkey meat present in the meat products with real-time PCR TaqMan probe technique.
The kit according to claim 1 wherein the primer-probe set can be applied to a heat treated meat product at 200° C. for 30min.
Dependencies are inferred from the claim text.