Method for Producing High Yield Attenuated Salmonella Strains
AssigneeVaximm AG
InventorsHeinz Lubenau, Holger Siede, Renate Janssen, Marco Springer
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Abstract
This invention relates to a novel method for growing attenuated mutant Salmonella typhi strains lacking galactose epimerase activity and harboring a recombinant DNA molecule. The method comprises the step of culturing said Salmonella typhi strain without adding glucose to the medium during the fermentation with a starting glucose amount that is depleted before reaching the stationary phase. The invention further relates to attenuated mutant Salmonella typhi strains obtainable by said method and to an attenuated mutant Salmonella typhi strain harboring a recombinant DNA molecule encoding a VEGF receptor protein for use as a vaccine.
Claims16
A method for growing an attenuated mutant strain of Salmonella typhi lacking galactose epimerase activity and comprising at least one copy of a recombinant DNA molecule comprising an expression cassette,
- comprising the step of culturing the strain in a buffered medium comprising peptone at approximately neutral starting pH value at fermentation scale,
- wherein the amount of glucose in the medium during the fermentation is adjusted such that the amount of glucose is reduced to zero before reaching the stationary phase.
An attenuated mutant strain of Salmonella typhi lacking galactose epimerase activity and comprising at least one copy of a recombinant DNA molecule comprising an expression cassette,
- obtainable by a method for growing the strain, comprising the step of
- culturing the strain in a buffered medium comprising peptone at approximately neutral starting pH at fermentation scale,
- wherein the amount of glucose in the medium during the fermentation is adjusted such that the amount of glucose is reduced to zero before reaching the stationary phase, preferably wherein no glucose is added to the medium during the fermentation and the starting amount of glucose is depleted before reaching the stationary phase.
An attenuated mutant strain of Salmonella typhi Ty21a comprising at least one copy of a recombinant DNA molecule comprising a eukaryotic expression cassette encoding a VEGF receptor protein, preferably a VEGF receptor protein selected from the group consisting of human VEGFR-2 and a homolog thereof that shares at least about 80% homology therewith, particularly wherein human VEGFR-2 has the amino acid sequence as found in SEQ ID NO 1, for use as a vaccine.
Dependencies are inferred from the claim text.