Amniotic Membrane Hydrogel and Methods of Making
AssigneeWake Forest University Health Sciences
InventorsSean V. Murphy, Aleksander Skardal, Anthony Atala
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Abstract
The present invention provides compositions and methods for wound healing and tissue regeneration. The compositions of the present invention comprise amniotic membrane of the placenta. In certain embodiments, the composition comprises amniotic membrane powder or solubilized amniotic membrane (SAM). In some aspects, the composition is cell-free and rich in cytokines, extracellular matrix proteins, and other components that improve tissue regeneration. In one aspect, the composition is a hydrogel scaffold that comprises amniotic membrane. The present invention reduces contraction and improves blood vessel development in regenerating tissue.
Claims30
1-38. (canceled)
A composition comprising amniotic membrane and a scaffold comprising a hydrogel comprising a biopolymer and a synthetic polymer.
A composition comprising amniotic membrane, a hydrogel comprising hyaluronan and gelatin, and a synthetic polymer.
A composition comprising amniotic membrane, thiolated hyaluronan, thiolated gelatin, and a pegylated synthetic polymer.
A method of making a composition comprising amniotic membrane and a scaffold comprising a hydrogel, the method comprising:
- a) washing amniotic membrane from a mammal;
- b) lyophilizing the washed amniotic membrane;
- c) grinding the lyophilized amiotic membrane to form a powder;
- d) forming a mixture of amniotic membrane powder, pepsin, and a solution;
- e) centrifuging the mixture to form a supernatant comprising solubilized amniotic membrane (SAM);
- f) adding the SAM to a hydrogel to form amniotic membrane comprising a hydrogel.
Dependencies are inferred from the claim text.