Particles comprising single stranded rna and double stranded rna for immunomodulation
AssigneeBioNTech SE
InventorSteve Pascolo
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Abstract
The present invention relates to chimeric particles comprising single stranded RNA (ssRNA), double stranded RNA (dsRNA) and at least one cationic agent, a pharmaceutical composition containing said particles and to a method of producing the same. The particles of the present invention are particularly useful as an immunostimulating medicament with a superlative pattern of immunostimulation.
Claims24
A particle comprising single stranded RNA (ssRNA) and double stranded RNA (dsRNA), wherein the RNA in the particle is associated with at least one cationic agent.
A method for the preparation of particles comprising the steps of:
- (a) providing an aqueous solution of ssRNA;
- (b) providing an aqueous solution of dsRNA;
- (c) providing an aqueous solution of protamine;
- and
- (d) combining the solutions obtained in steps (a) and (b) and mixing it with the solution obtained in (c).
A method for the preparation of particles comprising the steps of:
- (a) providing an aqueous solution of ssRNA at less than 5 mg/ml using a solution containing 0 to 125 mM electrolytes;
- (b) providing an aqueous solution of dsRNA at less than 5 mg/ml using a solution containing 0 to 125 mM electrolytes;
- (c) providing an aqueous solution of protamine at less than 5 mg/ml using a solution containing 0 to 125 mM electrolytes;
- and
- (d) combining the solutions obtained in steps (a) and (b) and adding the solution obtained in (c).
Dependencies are inferred from the claim text.