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US 2014/0348908 A1Published application
Methods and compositions involving mirna and mirna inhibitor molecules
AssigneeAsuragen, Inc.
InventorsDavid Brown, Lance Ford, Angie Cheng, Rich Jarvis, Mike Byrom, Dmitriy Ovcharenko, Eric Devroe, Kevin Kelnar
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Abstract
The present invention concerns methods and compositions for introducing miRNA activity or function into cells using synthetic nucleic acid molecules. Moreover, the present invention concerns methods and compositions for identifying miRNAs with specific cellular functions that are relevant to therapeutic, diagnostic, and prognostic applications wherein synthetic miRNAs and/or miRNA inhibitors are used in library screening assays.
Claims21
1-101. (canceled)
A synthetic RNA molecule comprising:
- a) a first 15-30 residue oligonucleotide having a sequence that is 80-100% identical to a mature microRNA sequence;
- b) a second 15-30 residue oligonucleotide having a sequence that is 60-100% identical to a sequence complementary to the mature microRNA sequence;
- and
- c) at least one of:
- i) a replacement group for the phosphate or the hydroxyl of the nucleotide at the 5′ end of the second oligonucleotide,
- ii) one or more sugar modifications in the first or last 1-6 residues of the second oligonucleotide, and
- iii) a noncomplementarity between one or more nucleotides in the last 1-5 residues at the 3′ end of the second oligonucleotide and the corresponding nucleotides of the first oligonucleotide.
4 more elements
Dependencies are inferred from the claim text.