Immunostimulatory compositions comprising liposome-encapsulated oligonucleotides and epitopes
AssigneeIndustry Academic Cooperation Foundation, Hallym University
InventorHyung-Joo KWON
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Abstract
The present invention relates to a composition for enhancing an immune response, an epitope having immunogenicity, screening and preparing method thereof, a antibody to peptide antigen and screening and preparing method thereof. The composition of the present invention may be effectively used for preventing or treating diverse immune-deficiency diseases such as cancer, influenza virus, hepatitis C virus and RSV (respiratory syncytial virus) by enhancing immune responses.
Claims6
1-30. (canceled)
A composition for enhancing an immune response comprising as an active ingredient (a) an immunostimulatory oligonucleotide and (b) an epitope encapsulated in a liposome, wherein the liposome is a mixture of CHEMS and DOPE, and wherein the molar ratio of CHEMS:DOPE in the mixture is 4.5:5.5-5.5:4.5
A method for enhancing an immune response in a subject in need thereof which comprises immunizing the subject with a composition comprising as an active ingredient (a) an immunostimulatory oligonucleotide and (b) an epitope encapsulated in a liposome, wherein the liposome is a mixture of CHEMS and DOPE, and wherein the molar ratio of CHEMS:DOPE in the mixture is 4.5:5.5-5.5:4.5
A screening method for an epitope having immunogenicity comprising the steps of:
- (a) encapsulating (i) an immunostimulatory oligonucleotide and (ii) a peptide as a candidate material for an epitope into a liposome, wherein the liposome is a mixture of CHEMS and DOPE, and wherein the molar ratio of CHEMS:DOPE in the mixture is 4.5:5.5-5.5:4.5;
- (b) immunizing a non-human animal with said liposome-encapsulated (i) the immunostimulatory oligonucleotide and (ii) the peptide as the candidate material for the epitope;
- and
- (c) analyzing the immune response of said immunized non-human animal.
A screening method for an antibody against a protein antigen comprising the steps of:
- (a) encapsulating (i) an immunostimulatory oligonucleotide and (ii) a peptide of the protein antigen as a candidate material for an epitope into a liposome, wherein the liposome is a mixture of CHEMS and DOPE, and wherein the molar ratio of CHEMS:DOPE in the mixture is 4.5:5.5-5.5:4.5;
- (b) immunizing a non-human animal with said liposome-encapsulated (i) the immunostimulatory oligonucleotide and (ii) the peptide as the candidate material for the epitope;
- (c) selecting a peptide epitope having immunogenicity by analyzing the immune response of said immunized non-human animal;
- (d) contacting said selected peptide epitope with the antibody of interest to be analyzed;
- (e) contacting the resultant of step (d) with said protein antigen;
- and
- (f) analyzing the binding of said protein antigen and the antibody of interest.
A method for preparing an antibody against a protein antigen comprising the steps of:
- (a) encapsulating (i) an immunostimulatory oligonucleotide and (ii) a peptide of the protein antigen as a candidate material for an epitope into a liposome, wherein the liposome is a mixture of CHEMS and DOPE, and wherein the molar ratio of CHEMS:DOPE in the mixture is 4.5:5.5-5.5:4.5
- (b) immunizing a non-human animal with said liposome-encapsulated (i) the immunostimulatory oligonucleotide and (ii) the peptide as the candidate material for the epitope;
- (c) selecting a peptide epitope having immunogenicity by analyzing the immune response of said immunized non-human animal;
- and
- (d) producing the antibody by immunizing a non-human animal with said selected peptide epitope.
Dependencies are inferred from the claim text.