Exosomes With Transferrin Peptides
AssigneeIsis Innovation Limited
InventorsMatthew Wood, Samira Lakhal-Littleton, Samir El Andaloussi
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Abstract
The present invention relates to exosomes comprising a transferrin targeting moiety on their surface, methods of producing them and to the use of such exosomes for delivering genetic material and/or biotherapeutic proteins or peptides or chemotherapeutic agents in vivo, in particular the use of such exosomes in methods of gene therapy or gene silencing, or delivery of other therapeutic agents.
Claims25
A composition comprising an exosome, wherein the exosome comprises a targeting moiety expressed on the surface of the exosome, and said targeting moiety is a transferrin (Tf) polypeptide or peptide.
A method of producing an exosome comprising a Tf polypeptide or Tf peptide targeting moiety expressed on the surface of the exosome comprising expressing a fusion protein comprising the targeting moiety and an exosomal transmembrane protein within a cell used to produce exosomes, wherein the expressed fusion protein is incorporated into the exosome as it is produced by the cell.
A polypeptide comprising an exosomal transmembrane protein, and a heterologous targeting Tf polypeptide or Tf peptide, wherein the targeting Tf polypeptide or Tf peptide binds to a Tf receptor (TfR) present on the surface of the cell to be targeted, and wherein when the polypeptide is present in an exosome, the targeting Tf polypeptide or Tf peptide is present on the surface of the exosome.
A polynucleotide construct encoding a polypeptide as defined in claim 22, wherein the construct comprises polynucleotide encoding a 5′ exosomal transmembrane signal sequence operatively linked to polynucleotide encoding the polypeptide of the invention.
Dependencies are inferred from the claim text.