Koi herpesvirus vaccine
AssigneeUniversity of Liege, Faculty of Veterinary Medicine
InventorAlain Vanderplasschen
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Abstract
The present invention relates to a recombinant Koi herpesvirus (KHV), methods for the production of such KHV, cells comprising such KHV and the use of such KHV as vector and in vaccines for the prevention and/or therapeutic treatment of a disease in fish caused by Koi herpesvirus in carp such as Cyprinus carpio carpio or Cyprinus carpio koi.
Claims17
1-15. (canceled)
A live recombinant Koi herpesvirus (KHV) in which Open Reading Frame 57 (ORF57) is deficient, resulting in a live recombinant KHV which is attenuated.
The live recombinant KHV of claim 1, that is deficient in at least one additional gene which contributes to virulence, but is not essential for replication.
The live recombinant KHV of claim 2,
- wherein said at least one additional gene is selected from the group consisting of thymidine kinase gene, ORF12:
- putative tumor necrosis factor (TNF) receptor gene, ORF16:
- putative G-protein coupled receptor (GPCR) gene, ORF134:
- putative Interleukin 10 homologue gene, and ORF 140:
- putative thymidylate kinase gene.
The live recombinant KHV of claim 1 that comprises a bacterial artificial chromosome (BAC) vector sequence.
The live recombinant KHV of claim 1 that further comprises a heterologous DNA fragment.
The live recombinant KHV of claim 1, which further comprises a heterologous DNA fragment that comprises a heterologous gene;
- wherein the heterologous gene is a gene encoding the G glycoprotein of rhabdovirus causing carp spring viraemia.
A vector for a heterologous DNA fragment comprising the live recombinant KHV of claim 1.
A cell comprising the recombinant KHV of claim 1.
A method for the production of infectious particles of recombinant Koi herpesvirus (KHV), comprising the steps of:
- (a) introducing the live recombinant KHV or a recombinant KHV DNA comprising the genome of the recombinant KHV into permissive eukaryotic cells;
- and
- (b) culturing said cell to produce the recombinant KHV or the recombinant KHV DNA.
A vaccine for the prevention or therapeutic treatment of a disease in fish caused by KHV comprising the live recombinant KHV of claim 1 or a recombinant KHV DNA comprising the genome of the recombinant KHV.
A vaccine for the prevention or therapeutic treatment of a disease in fish caused by rhabdovirus causing carp spring viraemia;
- wherein the vaccine comprises a pharmaceutically acceptable carrier and the live recombinant KHV of claim 1 comprising a heterologous gene, a recombinant KHV DNA comprising the genome of the recombinant KHV, which comprises a heterologous gene, or both said live recombinant KHV and said recombinant KHV DNA;
- wherein the heterologous gene encodes the G glycoprotein of rhabdovirus causing carp spring viraemia.
Dependencies are inferred from the claim text.