Isolated Brachyspira and Methods and Compositions for Expanding and Isolating Brachyspira
OwnerJohn Clare Samuel Harding, Janet Elizabeth Hill +2inventors
InventorsJohn Clare Samuel Harding, Janet Elizabeth Hill, Joseph Rubin, Manuel Chirino-Trejo
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Abstract
The disclosure provides isolated—, compositions comprising—and methods of culturing —a Brachyspira sp. Sask30446 organism. The method comprises inoculating a liquid media or solid media with a sample comprising the Brachyspira sp. Sask30446 organism and incubating the liquid media or solid media at a temperature between 25-44° C. under anaerobic conditions. Also provided is isolated Brachyspira sp. Sask30446 organism, compositions comprising Brachyspira sp. Sask30446 organism and methods and uses thereof.
Claims38
A method of culturing a Brachyspira sp. Sask30446 organism comprising at least 96% sequence identity to SEQ ID NOs:
- 7, 8 and 9;
- at least 92.3% sequence identity to SEQ ID NOs:
- 11 and 12;
- at least 97.5% sequence identity to SEQ ID NO:
- 25;
- at least 98.5% sequence identity to SEQ ID NO:26;
- at least 93.5% sequence identity to SEQ ID NO:27 and 31;
- at least 95.5% sequence identity to SEQ ID NO:28, 29 and 34;
- at least 99.5% sequence identity to SEQ ID NO:
- 30, 32 and 37 and at least 92.5% identity to SEQ ID NO:33, the method comprising inoculating an agar media and/or a liquid media with a sample comprising the Brachyspira sp. Sask30446 organism and incubating the agar media and/or the liquid media at a temperature between 25-44° C. under anaerobic conditions, wherein the liquid media comprises BHI, 1-20% blood product and 0.5% to 10% glucose, optionally wherein the agar media corn irises BAM, BJ, CVS or Blood agar media or a modified media thereof.
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An isolated Brachyspira sp. Sask30446 organism comprising one or more sequences of SEQ ID NOs:
- 7, 8 9, 11, 12, 25-34, 37;
- or at least 96% sequence identity to SEQ ID NOs:
- 7, 8 and 9, and at least 92.3% sequence identity to SEQ ID NOs:
- 11 and 12;
- at least 97.5% sequence identity to SEQ ID NO:
- 25;
- at least 98.5% sequence identity to SEQ ID NO:26;
- at least 93.5% sequence identity to SEQ ID NO:27 and 31;
- at least 95.5% sequence identity to SEQ ID NO:28, 29 and 34;
- at least 99.5% sequence identity to SEQ ID NO:
- 30, 32 and 37;
- and at least 92.5% sequence identity to SEQ ID NO:33;
- optionally wherein the Brachyspira sp. Sask30446 organism is packaged in a vial, such as a sterile vial and is optionally frozen, dessicated or refrigerated.
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A media composition comprising Brain Heart Infusion broth, 1 to 20% blood product and 0.5% to 10% glucose, optionally comprising 5% ovine blood, 5% fetal calf serum and 1% glucose, and optionally comprising at least one antibiotic.
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An isolated Brachyspira sp, comprising a DNA genome encoding SEQ ID NO:
- 11, 12, 26, 28 30, 32 and 34 and/or encoding polypeptides with at least 92.3% sequence identity with SEQ ID NO:
- 11 and 12;
- at least 98.5% sequence identity to SEQ ID NO:26;
- at least 95.5% sequence identity to SEQ ID NO:28 and 34;
- and at least 99.5% sequence identity to SEQ ID NO:
- 30 and 32.
Dependencies are inferred from the claim text.