Porcine circovirus type-2 (pcv2) subunit vaccine
AssigneeSBC Virbac Biotech Co., Ltd.
InventorsTsun-Yung Kuo, Hsu-Chung Gabriel Chen, Shu-Hsiang Yang, Yu-San Chen
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Abstract
A procine circovirus type-2 (PCV2) immunogenic composition includes an antigenic peptide. The antigenic peptide is a non-arginine-rich peptide of a PCV2 open reading frame 2 (ORF2) and/or a recombinant fusion protein having the non-arginine-rich peptide of the PCV2 ORF2, a PE peptide, and a KDEL signal peptide. The number of arginines of the non-arginine-rich peptide of the PCV2 ORF2 is not greater than half of the number of arginines of the arginine-rich domain of the N terminal of the PCV2 ORF2.
Claims17
A porcine circovirus type 2 (PCV2) immunogenic composition, comprising an antigenic peptide selected from at least one of:
- (a) a non-arginine-rich peptide of a PCV2 open reading frame 2 (ORF2), wherein the number of arginines of the non-arginine-rich peptide of the PCV2 ORF2 is not greater than half of the number of arginines of an arginine-rich domain of the PCV2 ORF2 at the N terminus of a full length peptide of the PCV2 ORF2;
- and
- (b) a recombinant fusion protein comprising, from the amino-terminus to the carboxyl-terminus of the recombinant fusion protein:
- a PE peptide having the sequence of SEQ ID NO:
- 35;
- the non-arginine-rich peptide of the PCV2 ORF2 of (a);
- and
- a KDEL signal peptide having the sequence of SEQ ID NO:
- 31.
A porcine circovirus type 2 (PCV2) immunogenic composition, comprising an antigenic peptide selected from at least one of:
- (a) an F2 peptide of a PCV2 open reading frame 2 (ORF2), wherein the F2 peptide of the PCV2 ORF2 comprises amino acid residues 79-156 at the N terminus of a full-length peptide of the PCV2 ORF2;
- and
- (b) a recombinant fusion protein comprising, from the amino-terminus to the carboxyl-terminus of the recombinant fusion protein:
- a PE peptide having the sequence of SEQ ID NO:
- 35;
- the F2 peptide of the PCV2 ORF2 of (a);
- and
- a KDEL signal peptide having the sequence of SEQ ID NO:
- 31.
A method for manufacturing an antigen fragment of PCV2, comprising:
- removing a DNA sequence encoding an arginine-rich domain of a PCV2 open reading frame 2 (ORF2) at the N terminus of a full length peptide of the PCV2 ORF2 from a full-length DNA sequence of the PCV2 ORF2 to obtain a DNA sequence encoding a non-arginine-rich domain of the PCV2 ORF2, wherein the number of arginines of the non-arginine-rich domain of the PCV2 ORF2 is not greater than half of the number of arginines of the arginine-rich domain of the PCV2 ORF2 at the N terminus of the full-length peptide of the PCV2 ORF2;
- and
- placing the DNA sequence encoding the non-arginine-rich domain of the PCV2 ORF2 in a biological expression system, and expressing the DNA sequence encoding the non-arginine-rich domain of the PCV2 ORF2 to obtain an antigen fragment of PCV2 immunogenic composition.
Dependencies are inferred from the claim text.