Use of complex forms of calmodulin-like skin protein clsp
AssigneeL'oreal
InventorsMark DONOVAN, Dominique Bernard
Life of this patent
Estimating the term…
Abstract
The invention also relates to the use of these complex forms for the purposes of screening for biological or chemical compounds capable of modulating a biological activity of said complex forms and/or for preparing and/or improving a pluristratified cell model.
Claims10
A method of screening for anti-ageing active agents, comprising:
- a) bringing at least one cell type containing at least one complex form of calmodulin-like skin protein, or CLSP, which derives at least in part from a polypeptide having an amino acid sequence encoded by a nucleic acid sequence represented completely or partly by SEQ ID NO 1 or an analogue thereof, or a fragment thereof, into contact with at least one test anti-ageing active agent, under conditions suitable for the expression of said complex form,
- b) determining a content of said complex form, and
- c) comparing said content determined in step b) with a content of said complex form determined in the absence of test chemical or biological compound.
A cosmetic method for characterizing the effectiveness of a cosmetic or therapeutic treatment intended to prevent and/or treat the signs of skin ageing linked to chronological ageing in an individual, comprising at least the qualitative or quantitative characterization of at least one complex form of calmodulin-like skin protein, or CLSP, which derives at least in part from a polypeptide having an amino acid sequence encoded by a nucleic acid sequence represented completely or partly by SEQ ID NO 1 or an analogue thereof, or a fragment thereof.
A nontherapeutic method for characterizing the effectiveness of a treatment capable of preventing and/or treating the signs of skin ageing in an individual, comprising:
- i. providing at least a first skin surface sample representative of said individual,
- ii. quantifying, in said sample, especially via an ELISA assay technique, at least one complex form of calmodulin-like skin protein, or CLSP, which derives at least in part from a polypeptide having an amino acid sequence encoded by a nucleic acid sequence represented completely or partly by SEQ ID NO 1 or an analogue thereof, or a fragment thereof,
- iii. repeating steps i. and ii. on a second skin surface sample representative of said individual, and
- iv. comparing the results obtained at the end of steps ii. and iii., in particular in order to deduce therefrom information relating to at least one effect of said treatment, said first and second skin surface samples corresponding to different treatment stages.
A method for screening for biological or chemical compound capable of modulating the increase in and/or the stability and/or the biological activity of at least one complex form of calmodulin-like skin protein, or CLSP, which derives at least in part from a polypeptide having an amino acid sequence encoded by a nucleic acid sequence represented completely or partly by SEQ ID NO 1 or an analogue thereof, or a fragment thereof, comprising contacting at least said complex form with at least one biological or chemical compound to be screened.
Dependencies are inferred from the claim text.