Compositions and methods for using cells to treat heart tissue
AssigneeMayo Foundation for Medical Education and Research
InventorsAndre Terzic, Atta Behfar
Life of this patent
Estimating the term…
Abstract
This document relates to compositions containing cardiogenic factors, to methods to obtain cells by culturing initial cells in the presence of such factors; and methods of administering the obtained cells to heart tissue.
Claims20
(canceled)
A composition for use in differentiation of stem cells into cardioprogenitor cells comprising:
- TGFβ-1, a BMP polypeptide, wherein the BMP polypeptide is selected from the group consisting of:
- BMP2 and BMP4;
- α-thrombin;
- FGF-2;
- IGF-1;
- Activin-A;
- Cardiotrophin;
- and Cardiogenol C.
A method for obtaining, from initial cells, differentiated cells expressing an elevated level of at least one of the mRNAs selected from the group consisting of MEF2c mRNA, MESP-1 mRNA, Tbx-5 mRNA, GATA4 mRNA, Flk-1 mRNA, GATA6 mRNA, Fog-1 mRNA, and combinations thereof, and/or having at least one polypeptide selected from the group consisting of Nkx2.5 polypeptides, MEF2C polypeptides, Tbx-5 polypeptides, FOG-2 polypeptides, GATA-4 polypeptides MESP-1 polypeptides, and combinations thereof, wherein said at least one polypeptide is associated with the nuclei of said differentiated cells, wherein said method comprises culturing initial cells in the presence of a composition comprising TGFβ-1, a BMP polypeptide selected from the group consisting of BMP2 and BMP4, α-thrombin, FGF-2, IGF-1, Activin-A, Cardiotrophin and Cardiogenol C.
A method for providing heart tissue with cardiomyocytes, wherein said method comprises administering, to said heart tissue, differentiated cells obtainable by contact with a composition comprising TGFβ-1, a BMP polypeptide selected from the group consisting of BMP2 and BMP4, α-thrombin, FGF-2, IGF-1, Activin-A, Cardiotrophin and Cardiogenol C.
Dependencies are inferred from the claim text.