Methods of separating lipids
AssigneeWaters Technologies Corporation
InventorsStephen Wong, Mark Ritchie
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Abstract
The invention provides novel, simple, reliable and effective methods for separating various lipids from biological samples. In certain embodiments, the invention provides HILIC-based UHPLC separation methods. In one embodiment, the methods of the invention offer an efficient and rapid separation of ether glycophospholipids (e.g., plasmalogens) from their diacyl or monoacyl counterparts within the same lipid class. In certain instances, the invention relates to the use of a Waters ACQUITY UPLC® system.
Claims17
A method of separating lipids of different classes from a biological sample, said method comprising steps of
- i) preparing the biological sample;
- ii) loading said biological sample onto an ultrahigh performance liquid chromatography (UHPLC) system, wherein said UHPLC system comprises a hydrophilic interaction chromatography (HILIC) column;
- iii) eluting said UHPLC system with an elution solvent;
- and
- iv) detecting lipids of different classes by using a mass spectrometer.
A method of separating ether glycophospholipids from a biological sample, said method comprising steps of
- i) preparing the biological sample;
- ii) loading said biological sample onto an ultrahigh performance liquid chromatography (UHPLC) system, wherein said UHPLC system comprises a hydrophilic interaction chromatography (HILIC) column;
- iii) eluting said UHPLC system with an elution solvent;
- and
- iv) detecting the ether glycophospholipids by using a mass spectrometer.
Dependencies are inferred from the claim text.