Method of separation of lipid and biological molecular species using high purity chromatographic materials comprising an ionizable modifier
AssigneeWaters Technologies Corporation
InventorsGiorgis Isaac, Stephen McDonald, Alan Millar, Kenneth Fountain, John Shockcor
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Abstract
The present invention provides methods of separating, profiling and analyzing molecular species contained within a biological sample using a high purity chromatographic material (HPCM) comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifiers with the proviso that when the ionizable modifier does not contain a Zwitterion, the ionizable modifier does not contain a quaternary ammonium ion moiety.
Claims34
An analytical method of separating components of a biological sample comprising a step of contacting said biological sample with a high purity chromatographic material comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifiers with the proviso that when the ionizable modifier does not contain a Zwitterion, the ionizable modifier does not contain a quaternary ammonium ion moiety.
An analytical method of separating components of a biological sample comprising a step of contacting said biological sample with a column chromatography device comprising
- a) a column having a cylindrical interior for accepting a stationary phase;
- and
- b) an immobilized stationary phase within said column, wherein said immobilized stationary phase comprises a high purity chromatographic material comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifiers with the proviso that when the ionizable modifier does not contain a Zwitterion, the ionizable modifier does not contain a quaternary ammonium ion moiety.
3-10. (canceled)
The method of claim 40, wherein the identification of the components is achieved by mass spectrometry, MALDI-MS, ESI-MS, nuclear magnetic resonance, infrared analysis, flow injection analysis, capillary electrochromatography, ultraviolet detection or a combination thereof.
Dependencies are inferred from the claim text.