Assay buffer, compositions containing the same, and methods of using the same
AssigneeMeso Scale Technologies, LLC.
InventorsMichael Tsionsky, Eli N. Glezer, Selen Stromgren, George Sigal, Jonathan K. Leland, Mark A. Billadeau, Svetlana Leytner, Mark Martin, Larry Helms
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Abstract
The invention relates to improved electrochemiluminescence assay methods for phosphorylated peptides or proteins employing phospho-specific antibodies and buffer compositions that are substantially free of inorganic phosphate.
Claims28
A kit suitable for use in an electrochemiluminescence method comprising, in one or more containers, a pH buffer and an ECL co-reactant, wherein said pH buffer is selected from the group consisting of glycylglycine, tris[hydroxymethyl]aminomethane or combinations thereof, and said ECL co-reactant comprises a tertiary amine.
A kit suitable for use in an electrochemiluminescence method comprising, in one or more containers, a pH buffer, an Ru- or Os-containing ECL label and an ECL co-reactant, said composition being substantially free of inorganic phosphate.
A kit suitable for use in an electrochemiluminescence method comprising, in one or more containers, a pH buffer and tripropylamine, said composition being substantially free of inorganic phosphate.
A kit suitable for use in an electrochemiluminescence method comprising, in one or more containers:
- (a) tripropylamine having a concentration ranging from 50 to 1000 mM and (b) a pH buffer having a concentration ranging from 50 to 1000 mM, said pH buffer being selected from the group consisting of glycylglycine, tris[hydroxymethyl]-aminomethane or combinations thereof.
A method of effecting a specific-binding assay, either qualitatively or quantitatively, in a composition comprising:
- (a) a pH buffer substantially free of inorganic phosphate and (b) a phosphor-specific antibody.
A method of effecting a specific-binding non-wash assay, either qualitatively or quantitatively, in a well having one or more assay domains with binding reagents immobilized thereon using a composition comprising a pH buffer substantially free of inorganic phosphate.
Dependencies are inferred from the claim text.