Assessment of oocyte competence by detecting spsb2 and/or tp53i3 gene expression
AssigneeGema Diagnostics, Inc.
InventorsDagan Wells, Pasquale Patrizio
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Abstract
Methods are provided for evaluating an oocyte for fertilization and implantation comprising analysis of gene expression in cumulus cells wherein the detected genes or polypeptides include at least SPSB2 and TP53I3. For example, methods are provided for determining whether a cumulus cell expresses, or does not express, one or more of a group of markers identified as differently expressed between cumulus cells associated with chromosomally normal oocytes and cumulus cells associated with chromosomally abnormal oocytes. Methods are provided for the detection of marker expression of differentially expressed genes at the RNA level, as well as at the protein level.
Claims69
A method of evaluating the competence of a mammalian oocyte for implantation or fertilization comprising:
- (i) obtaining a nucleic acid or polypeptide sample;
- (ii) determining the level of marker expression of at least one gene or corresponding polypeptide encoded thereby selected from the group of TP53I3 or SPSB2 in said sample;
- and
- (iii) comparing the level of marker expression TP53I3 or SPSB2 in the sample with a control or reference standard,
- wherein detecting differential marker expression between the sample and the control is indicative of the competence of an oocyte for implantation.
An array comprising at least SPSB2 and TP53I3 genes or SPSB2 and TP53I3 nucleic acids, primers, polypeptides or antibodies which specifically detect, amplify, or bind to SPSB2 and TP53I3 nucleic acids or polypeptides.
Dependencies are inferred from the claim text.