Yeast recombinant cell capable of producing gdp-fucose
AssigneeSilab
InventorsChristophe Javaud, Christelle Arico, Christine Bonnet
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Abstract
A yeast strain expressing a bi-functional fucokinase/GDP-L-fucose pyrophosphorylase enzyme and capable of producing GDP-L-fucose in vivo is provided. Also provided are yeast cells which express a GDP-L-fucose transporter and/or a fucosyl transferase with the bi-functional enzyme. In addition, the said yeast contains one or more expression cassettes for fusion proteins of heterologous glycosylation pathway and an ER/Golgi retention sequence. Finally, the invention also provides a method for producing recombinant target glycoproteins.
Claims25
A genetically modified yeast comprising at least one cassette for expressing a bi-functional fucokinase/GDP-L-fucose pyrophosphorylase enzyme.
Dependencies are inferred from the claim text.