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US 2014/0304859 A1Published application
Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsYe Liu, Yu Zhang, Junxin Duan, Lan Tang
Life of this patent
Estimating the term…
Abstract
Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims16
1-18. (canceled)
An isolated polypeptide having endoglucanase activity, selected from the group consisting of:
- (a) a polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO:
- 4, a polypeptide having at least 71% sequence identity to the mature polypeptide of SEQ ID NO:
- 14, a polypeptide having at least 65% sequence identity to the mature polypeptide of SEQ ID NO:
- 18 or the mature polypeptide of SEQ ID NO:
- 24, a polypeptide having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
- 10, the mature polypeptide of SEQ ID NO:
- 22, or the mature polypeptide of SEQ ID NO:
- 22, a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 6, the mature polypeptide of SEQ ID NO:
- 8, or the mature polypeptide of SEQ ID NO:
- 16, a polypeptide having at least 76% sequence identity to the mature polypeptide of SEQ ID NO:
- 12, or a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 20;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 3, the mature polypeptide coding sequence of SEQ ID NO:
- 13, the mature polypeptide coding sequence of SEQ ID NO:
- 1, the mature polypeptide coding sequence of SEQ ID NO:
- 5, the mature polypeptide coding sequence of SEQ ID NO:
- 7, the mature polypeptide coding sequence of SEQ ID NO:
- 11, the mature polypeptide coding sequence of SEQ ID NO:
- 15, the mature polypeptide coding sequence of SEQ ID NO:
- 21, or the mature polypeptide coding sequence of SEQ ID NO:
- 23, or the cDNA sequence thereof (ii), the mature polypeptide coding sequence of SEQ ID NO:
- 9, the mature polypeptide coding sequence of SEQ ID NO:
- 17, or the mature polypeptide coding sequence of SEQ ID NO:
- 19, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3, a polypeptide encoded by a polynucleotide having at least 71% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 13, a polypeptide encoded by a polynucleotide having at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 17 or the mature polypeptide coding sequence of SEQ ID NO:
- 23, a polypeptide encoded by a polynucleotide having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 9, the mature polypeptide coding sequence of SEQ ID NO:
- 21 or the mature polypeptide coding sequence of SEQ ID NO:
- 1, a polypeptide encoded by a polynucleotide having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5, the mature polypeptide coding sequence of SEQ ID NO:
- 7 or the mature polypeptide coding sequence of SEQ ID NO:15, a polypeptide encoded by a polynucleotide having at least 76% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 11, or a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 19;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 4, a variant of the mature polypeptide of SEQ ID NO:
- 2, a variant of the mature polypeptide of SEQ ID NO:
- 6, a variant of the mature polypeptide of SEQ ID NO:
- 8, a variant of the mature polypeptide of SEQ ID NO:
- 10, a variant of the mature polypeptide of SEQ ID NO:
- 12, a variant of the mature polypeptide of SEQ ID NO:
- 14, a variant of the mature polypeptide of SEQ ID NO:
- 16, a variant of the mature polypeptide of SEQ ID NO:
- 18, a variant of the mature polypeptide of SEQ ID NO:
- 20, a variant of the mature polypeptide of SEQ ID NO:
- 22, or a variant of the mature polypeptide of SEQ ID NO:
- 24, comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has endoglucanase activity.
50 more elements
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 90% sequence identity to the sequence of amino acids 80-383 of SEQ ID NO:
- 4, a catalytic domain having at least 71% sequence identity to the sequence of amino acids 45-346 of SEQ ID NO:
- 14, a catalytic domain having at least 65% to the sequence of amino acids 61-448 of SEQ ID NO:
- 18 or the amino acids 60-444 of SEQ ID NO:
- 24, a catalytic domain having at least 70% sequence identity to the sequence of amino acids 49-416 of SEQ ID NO:
- 10, the amino acids 80-404 of SEQ ID NO:
- 22 or the amino acids 22-390 of SEQ ID NO:
- 2, a polypeptide having at least 75% sequence identity to the sequence of amino acids 20-342 of SEQ ID NO:
- 6, the amino acids 26-382 of SEQ ID NO:
- 8 or the amino acids 26-418 of SEQ ID NO:
- 16, a catalytic domain having at least 76% sequence identity to the sequence of amino acids 19-324 of SEQ ID NO:
- 12, or a catalytic domain having at least 80% sequence identity to the sequence of amino acids 28-414 of SEQ ID NO:
- 20;
- (b) a catalytic domain encoded by a polynucleotide that hybridizes under low conditions with (i) nucleotides 238 to 1441 of SEQ ID NO:
- 3, nucleotides 133 to 1316 of SEQ ID NO:
- 13, nucleotides 64 to 1254 of SEQ ID NO:
- 1, nucleotides 58 to 1300 of SEQ ID NO:
- 5, nucleotides 76 to 1230 of SEQ ID NO:
- 7, nucleotides 55 to 1333 of SEQ ID NO:
- 11, nucleotides 76 to 1452 of SEQ ID NO:
- 15, nucleotides 349 to 1535 of SEQ ID NO:
- 21, or nucleotides 178 to 1332 of SEQ ID NO:
- 23, or the cDNA sequence thereof (ii), nucleotides 145 to 1248 of SEQ ID NO:
- 9, nucleotides 181 to 1344 of SEQ ID NO:
- 17, or nucleotides 82 to 1242 of SEQ ID NO:
- 19, or (iii) the full-length complement of (i) or (ii);
- (c) a catalytic domain encoded by a polynucleotide having at least 90% sequence identity to the sequence of nucleotides 238 to 1441 of SEQ ID NO:
- 3, a catalytic domain encoded by a polynucleotide having at least 71% sequence identity to the sequence of nucleotides 133 to 1316 of SEQ ID NO:
- 13, a catalytic domain encoded by a polynucleotide having at least 65% sequence identity to the sequence of nucleotides 181 to 1344 of SEQ ID NO:
- 17 or the nucleotides 178 to 1332 of SEQ ID NO:
- 23, a catalytic domain encoded by a polynucleotide having at least 70% sequence identity to the sequence of nucleotides 145 to 1248 of SEQ ID NO:
- 9, the nucleotides 349 to 1535 of SEQ ID NO:
- 21 or the nucleotides 64 to 1254 of SEQ ID NO:
- 1, a catalytic domain encoded by a polynucleotide having at least 75% sequence identity to the sequence of nucleotides 58 to 1300 of SEQ ID NO:
- 5, the nucleotides 76 to 1230 of SEQ ID NO:
- 7 or the nucleotides 76 to 1452 of SEQ ID NO:
- 15, a catalytic domain encoded by a polynucleotide having at least 76% sequence identity to the sequence of nucleotides 55 to 1333 of SEQ ID NO:
- 11, or a catalytic domain encoded by a polynucleotide having at least 80% sequence identity to the sequence of nucleotides 82 to 1242 of SEQ ID NO:
- 19;
- (d) a variant of amino acids 80 to 383 of SEQ ID NO:
- 4, amino acids 45 to 346 of SEQ ID NO:
- 14, amino acids 22 to 390 of SEQ ID NO:
- 2, amino acids 20 to 342 of SEQ ID NO:
- 6, amino acids 26 to 382 of SEQ ID NO:
- 8, amino acids 49 to 416 of SEQ ID NO:
- 10, amino acids 19 to 324 of SEQ ID NO:
- 12, amino acids 26 to 418 of SEQ ID NO:
- 16, amino acids 61 to 448 of SEQ ID NO:
- 18, amino acids 28 to 414 of SEQ ID NO:
- 20, amino acids 80 to 404 of SEQ ID NO:
- 22, or amino acids 60 to 444 of SEQ ID NO:
- 24, comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the catalytic domain of (a), (b), (c), or (d), which has endoglucanase activity.
51 more elements
An isolated polypeptide comprising a carbohydrate binding domain selected from the group consisting of:
- (a) a carbohydrate binding domain having at least 90% sequence identity to amino acids 17 to 52 of SEQ ID NO:
- 4, at least 71% sequence identity to amino acids 442 to 474 of SEQ ID NO:
- 14, or at least 70% sequence identity to amino acids 22 to 50 of SEQ ID NO:
- 22;
- (b) a carbohydrate binding domain encoded by a polynucleotide that hybridizes under low, medium, medium-high, high, or very high stringency conditions with (i) nucleotides 49 to 156 of SEQ ID NO:
- 3, nucleotides 1602 to 1700 of SEQ ID NO:
- 13, or nucleotides 64 to 261 of SEQ ID NO:
- 21, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a carbohydrate binding domain encoded by a polynucleotide having at least 90% sequence identity to the sequence of nucleotides 49 to 156 of SEQ ID NO:
- 3, at least 71% sequence identity to the sequence of nucleotides 1602 to 1700 of SEQ ID NO:
- 13, or at least 70% sequence identity to the sequence of nucleotides 64 to 261 of SEQ ID NO:
- 21;
- (d) a variant of amino acids 17 to 52 of SEQ ID NO:
- 4, amino acids 442 to 474 of SEQ ID NO:
- 14 or amino acids 22 to 50 of SEQ ID NO:
- 22, comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the carbohydrate binding domain of (a), (b), (c) or (d) that has carbohydrate binding activity.
15 more elements
Dependencies are inferred from the claim text.