Method and apparatus for aseptic transfer of biological material
AssigneeKing Abdulaziz City for Science and Technology (KACST)
InventorMOHAMMAD AHMED KHIYAMI
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Abstract
The present application discloses a novel biological material transfer apparatus and methods of using the same. The apparatus is made up of double walled barrel shaped tip chamber to house either the single tip or multiple tips of fixed size and a unique ball head to transfer the biological material from one location to another location in an aseptic method. The tip has a fixed shape and size and a ball head which makes the process of picking and transferring of biological material easy, safe and convenient to use with large scale samples. The apparatus is further equipped with an ultra violet light which sterilize the tips just before their use making the entire process of transferring of biological material highly aseptic.
Claims20
A biological material transfer apparatus, comprising:
- a barrel shaped tip chamber having a double wall to house a tip, wherein the tip is at least one of a single tip and multiple tips, wherein the barrel shaped tip chamber has an inner wall and an outer wall;
- the tip having a specific size and ball head, wherein the ball head is used to pick and transfer the biological material from one location to another location;
- a score lid to open and load the tip in the barrel shaped tip chamber;
- an eject button to push the tip into the barrel shaped tip chamber and out of the barrel shaped tip chamber for transfer of biological material;
- an ultra violet (UV) light having a specific wavelength to sterilize the barrel shaped tip chamber and ball head tip after being switched on for a specific time and having a specific strength.
An aseptic method to perform a biological material transfer, comprising:
- irradiating a barrel shaped tip chamber of the biological material transfer apparatus using an ultra violet light for a specific time and a specific wavelength;
- autoclaving a tip to make a sterile tip and loading on the barrel shaped tip chamber by bringing a score lid to open position;
- lowering the sterile tip into the barrel shaped tip chamber by pushing an eject button till a ball head reaches a distal end;
- re-irradiating the sterile tip by switching on the ultra violet light for the specific time and specific wavelength;
- picking up a biological material from one location and transfer the biological material to another location;
- and
- discarding the sterile tip after use by pushing the eject button.
An aseptic method to perform a biological material transfer, comprising;
- opening the proximal end of the tip chamber by tilting the eject button manually;
- disinfecting the proximal end before loading the tips;
- loading the required number of sterile tips in the tip chamber;
- and
- closing the proximal end by pushing the eject button back to its first position.
Dependencies are inferred from the claim text.