Absorbent paper and use thereof for breast cancer detection
AssigneeAtossa Genetics, Inc.
InventorsSteven C. Quay, Shu-Chih Chen
Life of this patent
Estimating the term…
Abstract
Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided.
Claims60
A system for analyzing a nipple aspirate fluid (NAF) sample from an individual in need thereof comprising:
- a. an absorbent paper for absorbing the nipple aspirate fluid sample, the absorbent paper comprising microcellulose, mixed cellulose ester paper, or nitrocellulose, wherein the absorbent paper is sized to cover a nipple;
- and
- b. a microscope for visualizing total protein of the nipple aspirate fluid sample, counting the number cells of the nipple aspirate fluid sample, or a combination thereof.
A non-transitory computer-readable storage media encoded with a computer program including instructions executable by a computer processing device to create an application, the application comprising:
- (a) a software module configured to apply a model or algorithm for analyzing the total protein content and/or the number of cells of a nipple aspirate fluid sample absorbed onto an absorbent paper that is sized to cover a nipple;
- and
- (b) a software module configured to designate a treatment regimen for the individual.
An absorbent paper that is sized to cover a nipple, comprising microcellulose, mixed cellulose ester paper or nitrocellulose.
A method of identifying risk of developing breast cancer in an individual in need thereof, comprising:
- a) detecting the total amount of protein and/or number of cells in a nipple aspirate fluid sample absorbed onto an absorbent paper, wherein said absorbent paper comprises microcellulose, mixed cellulose ester paper or nitrocellulose;
- and
- b) identifying the risk for developing breast cancer based on the total amount of protein and/or the number of cells of the nipple aspirate fluid sample.
A composition comprising nipple aspirate fluid (NAF) and an absorbent paper that is sized to cover a nipple, comprising microcellulose, mixed cellulose ester paper or nitrocellulose.
A system for analyzing a nipple aspirate fluid (NAF) sample from an individual in need thereof comprising:
- a. an absorbent paper for absorbing the nipple aspirate fluid sample, the absorbent paper comprising microcellulose, mixed cellulose ester paper, or nitrocellulose, wherein the absorbent paper is sized to cover a nipple;
- and
- b. a breast pump device.
A system for analyzing a nipple aspirate fluid (NAF) sample from an individual in need thereof comprising:
- a. an absorbent paper for absorbing the nipple aspirate fluid sample, the absorbent paper comprising microcellulose, mixed cellulose ester paper, or nitrocellulose, wherein the absorbent paper is sized to cover a nipple;
- and
- b. colloidal metal stain for determining the total protein of the nipple aspirate fluid sample.
Dependencies are inferred from the claim text.