Antibody and antibody mimetic for visualization and ablation of endogenous proteins
AssigneeUniversity of Southern California
InventorsDon B. Arnold, Richard W. Roberts, Garrett G. Gross, Rudy Mora, Jason Junge
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Abstract
Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.
Claims45
A polynucleotide comprising (1) a nucleic acid sequence encoding a fusion polypeptide comprising (a) a binding moiety and (b) a transcription factor and (2) a transcription regulatory element operatively linked to the nucleic acid sequence, wherein the transcription regulatory element regulates the transcription of the binding moiety and the transcription factor regulates the activity of the transcription regulatory element.
A method for labeling an endogenous protein in a cell, comprising contacting the cell with a polynucleotide comprising (1) a nucleic acid sequence encoding a fusion polypeptide comprising (a) a binding moiety, (b) a reporter and (c) a transcription factor and (2) a transcription regulatory element operatively linked to the nucleic acid sequence, wherein the transcription regulatory element regulates the transcription of the binding moiety and the transcription factor regulates the activity of the transcription regulatory element, and wherein the binding moiety specifically recognizes the endogenous protein.
A polynucleotide comprising a nucleic acid sequence encoding a fusion polypeptide comprising (a) a binding moiety and (b) a protein degradation signal.
A composition comprising (1) a first polynucleotide encoding a fusion polypeptide comprising a binding moiety and (2) a second polynucleotide encoding a fusion polypeptide comprising a target peptide sequence and a cell localization domain, wherein the binding moiety specifically recognizes the target peptide sequence.
A kit comprising (1) a first polynucleotide encoding a fusion polypeptide comprising a binding moiety and a reporter and (2) a second polynucleotide encoding a fusion polypeptide comprising a target peptide sequence and a cell localization domain, wherein the binding moiety specifically recognizes the target peptide sequence.
(canceled)
Dependencies are inferred from the claim text.