Compositions and methods for nucleic acid extraction
AssigneeAbbott Molecular Inc.
InventorGerard J. Gundling
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Abstract
Compositions and methods for the efficient extraction, enrichment and isolation of nucleic acids from fresh, fixed or fixed and embedded cells, tissues, biological materials and cellular source material.
Claims32
1) A method of extracting nucleic acid from cellular source material, said method comprising:
- a) providing i) cellular source material and ii) an aqueous extraction solution comprising one or more amine monomers;
- b) contacting said cellular source material with said extraction solution resulting in lysis of the cellular material and extraction of the nucleic acids.
9) The method of claim 9, wherein the concentration of said detergent is about 8% to about 15%.
22) A composition comprising an aqueous extraction solution suitable for the extraction of nucleic acids from cellular source material, said composition comprising one or more amine monomers;
- one or more chaotropic reagents, one or more detergents and one or more organic solvents.
28) The composition of claim 29, wherein the concentration of said detergent is about 8% to about 15%.
30) The composition of claim 31, wherein the concentration of said alcohol is about 15% to about 25%.
Dependencies are inferred from the claim text.