Protein purification using displacement chromatography
AssigneeAbbVie Inc.
InventorsChen Wang, Germano Copploa
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Abstract
Disclosed herein are compositions and methods for the isolation and purification of proteins from a sample. In particular, the present invention relates to compositions and methods for isolating and purifying proteins incorporating a displacement chromatographic step. The present invention is also directed toward pharmaceutical compositions comprising one or more antibodies purified by a method described herein.
Claims22
A method for reducing process-related impurities and/or product-related substances in a preparative scale sample of a protein of interest comprising at least one process-related impurities and/or product-related substance, the method comprising:
- (a) contacting the sample to a chromatography media under conditions wherein the protein of interest binds to the chromatography media;
- (b) displacing the protein of interest bound to the chromatography media with a displacer buffer comprising at least one displacer molecule;
- and
- (c) collecting a chromatography sample,
- wherein the chromatography sample comprises a reduced amount of process-related impurities and/or product-related substances.
A composition comprising a protein of interest and a reduced level of protein aggregates.
A composition comprising a protein of interest and a reduced level of protein fragments.
Dependencies are inferred from the claim text.