Epigenetic biomarker adamts9 for diagnosis and prognosis of gastric cancer
AssigneeThe Chinese University of Hong Kong
InventorsJUN YU, Joseph Jao Yiu Sung
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Abstract
The present invention provides a method for diagnosing and determining prognosis of gastric cancer in a subject by detecting suppressed expression of the ADAMTS9 gene, which in some cases is due to elevated methylation level in the genomic sequence of this gene. A kit and device useful for such a method are also provided. In addition, the present invention provides a method for treating gastric cancer by increasing ADAMTS9 gene expression or activity.
Claims20
A method for detecting gastric cancer in a subject, comprising the steps of:
- (a) measuring expression level of ADAMTS9 in a sample taken from the subject, and
- (b) comparing the expression level obtained in step (a) with a standard control,
- wherein a decrease in the expression level of ADAMTS9 when compared with the standard control indicates the subject having gastric cancer.
A method for detecting gastric cancer in a subject, comprising the steps of:
- (a) treating a sample taken from the subject with an agent that differentially modifies methylated and unmethylated DNA;
- and
- (b) determining whether each CpG in a CpG-containing genomic sequence is methylated or unmethylated, wherein the CpG-containing genomic sequence is at least a segment of SEQ ID NO:1, 6 or 7 and comprising at least one CpG,
- wherein presence of one methylated CpG in the CpG-containing genomic sequence indicates the subject having gastric cancer.
A kit for detecting gastric cancer in a subject, comprising (1) a standard control that provides an average amount of ADAMTS9 protein or ADAMTS9 mRNA;
- and (2) an agent that specifically and quantitatively identifies ADAMTS9 protein or ADAMTS9 mRNA.
A method for inhibiting growth of a gastric cancer cell, comprising contacting the gastric cancer cell with an effective amount of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:5 or a nucleic acid comprising a polynucleotide sequence encoding SEQ ID NO:5.
An isolated nucleic acid having the nucleotide sequence at least 95% identical to a segment of about 20-100 contiguous nucleotides of SEQ ID NO:1-4, 6-14 or 15 or complement thereof.
Dependencies are inferred from the claim text.