Back to results
US 2014/0274894 A1Published application
Methods for making cytokine compositions from tissues using non-centrifugal methods
AssigneeBiomet Biologics, LLC
InventorsMichael D. LEACH, Jennifer E. WOODELL-MAY, Joel C. HIGGINS, Krista O'SHAUGHNESSEY
Life of this patent
Estimating the term…
Abstract
Non-centrifugal methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The solution rich in IL-1ra can also include at least one of sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-β1, and sIL-1 RII.
Claims26
A method for generating a solution rich in interleukin-1 receptor antagonist (IL-1ra) comprising:
- (a) obtaining a cytokine cell suspension by separating cytokine-producing cells from a tissue comprising cytokine-producing cells using a non-centrifugal process;
- (b) contacting the liquid volume comprising cytokine-producing cells with a solid extraction material;
- and
- (c) separating the liquid volume from the solid extraction material to obtain the solution rich in IL-1ra.
1 more element
A method for treating a disorder mediated by interleukin-1 (IL-1) in a mammalian subject, the method comprising:
- (a) mixing a volume of cytokine-producing cells with a liquid medium to form a cytokine cell suspension, wherein the cytokine-producing cells are selected from the group consisting of white blood cells, adipose stromal cells and mesenchymal stromal cells, and mixtures thereof;
- (b) contacting the cytokine cell suspension with polyacrylamide beads for from about 30 minutes to about 2 hours;
- and
- (c) separating liquid from the polyacrylamide beads to obtain a solution rich in IL-1ra.
1 more element
Dependencies are inferred from the claim text.