miRNA BIOMARKERS OF LUNG DISEASE

A method of characterizing a lung disease in a patient as cancerous or benign, comprising the steps of:

• a. reverse transcribing a miRNA in a serum sample;
• b. amplifying the miRNA;
• c. measuring the level of the miRNA;
• d. detecting whether the level of the miRNA is elevated or reduced,
• thereby characterizing the lung disease as cancerous if the miRNA is elevated or reduced,
• wherein the miRNA is chosen from miR-210, miR-155, miR-142-5p, miR-181d, miR-27b, miR-15b, miR-126, miR-21, miR-205, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, let-7i, miR-10a, miR-100b, miR-130b, miR-132, miR-133b, miR-139, miR-143, miR-152, miR-17-5p, miR-193, miR-194, miR-195, miR-196b, miR-199a*, miR-19b, miR-202, miR-204, miR-206, miR-20b, miR-214, miR-221, miR-27a, miR-296, miR-29a, miR-301, miR-324-3p, miR-324-5p, miR-339, miR-346, miR-365, miR-378, miR-422a, miR-432, miR-485-3p, miR-496, miR-497, miR-505, miR-518b, miR-525, miR-566, miR-605, miR-638, miR-660, miR-93, miR-101, miR-106a, miR-106b, miR-125a, miR-140, miR-145, miR-146a, miR-146b, miR-150, miR-151, miR-15a, miR-181a, miR-181b, miR-185, miR-186, miR-190, miR-191, miR-222, miR-23a, miR-24, miR-26a, miR-26b, miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-30e-5p, miR-320, miR-335, miR-340, miR-342, miR-345, and miR-375.

A method of characterizing a lung disease in a patient, comprising the steps of:

• a. reverse transcribing a first miRNA and a second miRNA in a serum sample;
• b. amplifying the first miRNA and the second miRNA;
• c. measuring the levels of the first miRNA and the second miRNA;
• d. detecting whether the levels of the first miRNA and the second miRNA are elevated or reduced,
• thereby characterizing the lung disease in the patient,
• wherein the first and second miRNA are chosen from miR-210, miR-155, miR-142-5p, miR-181d, miR-27b, miR-15b, miR-126, miR-21, miR-205, let-7a, let-7b, let-7d, let-7f, let-7g, let-7i, miR-101, miR-106a, miR-106b, miR-125a, miR-126*, miR-130b, miR-132, miR-133b, miR-140, miR-145, miR-146a, miR-146b, miR-148b, miR-150, miR-151, miR-15a, miR-181a, miR-181b, miR-185, miR-186, miR-190, miR-191, miR-193a, miR-199a*, miR-202, miR-214, miR-222, miR-23a, miR-24, miR-26a, miR-26b, miR-27a, miR-29b, miR-301, miR-30a-5p, miR-30b, miR-30c, miR-30d, miR-30e-5p, miR-320, miR-324-3p, miR-326, miR-335, miR-340, miR-342, miR-345, miR-346, miR-34a, miR-374, miR-375, miR-378, miR-422a, miR-422b, miR-425, miR-486, miR-496, miR-518b, miR-660, miR-7, miR-92, miR-93, miR-98, miR-99a, and miR-99b.