Chlamydia-specific cd8+ t cells and methods of isolating
OwnerRaymond M. Johnsoninventor
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Abstract
Isolated, Chlamydia-specific, IL-13 expressing CD8+ T cell clones are provided for understanding the biology of Chlamydia infection and screening of therapeutics. Furthermore, methods are provided for isolating CD8+ T cells comprising the steps of infecting or identifying a naturally infected mammal with at least one species of Chlamydia causing bacteria, allowing the bacteria to clear or for a T cell immune response to the natural infection, collecting immune splenocytes from the mammal, providing at least one antigen from a Chlamydia-specific bacteria, and expanding and depleting the CD4+ T cell population or purifying the CD8 T cell population to isolate IL-13 expressing CD8+ T cell clones.
Claims20
A method for isolating Chlamydia-specific CD8+ T cells comprising the steps of:
- recovering immune splenocytes from a mammal previously infected with at least one species of a Chlamydia-causing bacteria;
- providing the immune splenocytes with at least one antigen to the Chlamydia-causing bacteria;
- culturing a population of the immune splenocytes under conditions which bring about an increase in the number of CD8+ T cells in the a population and utilizes immune antigen presenting cells;
- isolating CD8+ T cells from the population;
- and
- deriving individual CD8+ T cell clones from the isolated CD8+ T cells, wherein the clones comprise interleukin-13 expressing CD8+ T cell clones.
An isolated, Chlamydia-specific, interleukin-13 expressing CD8+ T cell clone cultured from a population of immune splenocytes utilizing immune antigen presenting cells.
A method for determining a T cell subset comprising the step of identifying a Chlamydia-specific, interleukin-13 expressing CD8+ T cell subset applicable to the diagnosis and/or treatment of one or more disease states.
Dependencies are inferred from the claim text.