Affinity reagents and methods for detection, purification, and proteomic analysis of methylated proteins
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsScott M. Carlson, Or Gozani, Kaitlyn E. Moore
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Abstract
Methyl-lysine affinity reagents created by engineering the 3×MBT methyl-lysine binding domain repeat of lethal (3) malignant brain tumor-like protein 1 (L3MBTL1) are disclosed. In particular, the invention relates to affinity reagents and affinity chromatography media comprising the 3×MBT domain repeat and methods of using such affinity reagents in detection, purification, and proteomic profiling of methylated proteins and peptides.
Claims58
An affinity reagent that binds to a methylated lysine residue, said affinity reagent comprising one or more engineered 3×MBT domains of one or more L3MBTL1 proteins, wherein each 3×MBT domain consists of residues of a L3MBTL1 protein corresponding to amino acids 190 to 530 of human L3MBTL1 numbered relative to the reference sequence of SEQ ID NO:2.
The affinity reagent of claim 2, wherein the affinity reagent comprises contiguous residues 190 to 530 of the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.