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US 2014/0274779 A1Published application
Multiplex allele detection
AssigneeAbbott Molecular Inc.
InventorsShihai Huang, Hong Su
Life of this patent
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Abstract
Provided herein is technology relating to nucleic acid detection and particularly, but not exclusively, to methods and compositions for the simultaneous detection of multiple nucleic acids.
Claims19
A method for detecting a nucleic acid in a sample, the method comprising:
- 1) contacting a sample comprising a nucleic acid with:
- a) a primer in a quenched state;
- b) a primer and a probe in a quenched state;
- or
- c) a primer and a probe in an unexcited state;
- and
- 2) detecting:
- a) a signal from the primer in a detectable state if:
- i) the primer hybridizes to the nucleic acid;
- or
- ii) the primer hybridizes to the nucleic acid and the primer is incorporated into an amplicon;
- or
- b) a signal from the primer if the probe hybridizes to a complement of the nucleic acid comprising the primer,
- wherein the nucleic acid comprises a BRAF gene or a portion of a BRAF gene and the nucleic acid is detected in the sample when the signal is detected.
11 more elements
A kit for detecting a BRAF allele comprising:
- 1) a detection reagent for detecting one or more BRAF alleles, wherein the detection reagent comprises one of the following:
- a) a stem-loop primer comprising a fluorophore and a quencher;
- b) a double-stranded linear primer comprising an allele-specific single-stranded primer comprising a fluorophore and a complementary quenching oligonucleotide;
- c) a double-stranded linear primer comprising an allele-specific single-stranded primer comprising a fluorophore and a complementary oligonucleotide;
- d) an allele-specific primer comprising a fluorophore and a double-stranded probe comprising a probe strand comprising a second fluorophore and a quencher oligonucleotide;
- e) an allele-specific primer comprising a fluorophore and a double-stranded probe comprising a probe strand comprising a second fluorophore;
- f) an allele-specific primer comprising a fluorophore and a single-stranded probe comprising a second fluorophore;
- or
- g) an allele-specific primer comprising a fluorophore and a stem-loop probe comprising a second fluorophore and a quencher;
- and
- 2) a control nucleic acid comprising a nucleotide sequence from a BRAF gene or from a portion of a BRAF gene.
8 more elements
Dependencies are inferred from the claim text.