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US 2014/0274778 A1Published application
Methods and systems for multiplex assays
AssigneePlexbio Co., Ltd.
InventorsDean TSAO, Chin-Shiou HUANG
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Abstract
Disclosed herein are compositions comprising beads with unique identifiers for storing information about a multiplex assay as well as methods for using the same in multiplex chemical and biological assays.
Claims31
A method for conducting a multiplex assay comprising the steps of:
- a) contacting a sample with beads in an assay system, wherein each bead in the system comprises an identifier;
- wherein the system comprises (i) at least one bead with an identifier that is recognized by an imaging processor as a specific assay, and (ii) a plurality of beads, wherein each bead comprises a capture agent immobilized on the surface of the bead that specifically binds to an analyte;
- and simultaneously or sequentially, in any order:
- b) identifying the identifier that is recognized by the imaging processor as the specific assay;
- and
- c) detecting the binding of the analyte in the sample by analyzing the binding of the analyte to the capture agent immobilized on the surface of the bead based on the identification of the identifier as the specific assay.
3 more elements
A kit for conducting a multiplex assay comprising:
- at least one bead with an identifier that is recognized by an imaging processor as a specific assay;
- and one or more of
- a) a plurality of beads, wherein each bead comprises i) an identifier, and ii) a capture agent immobilized on the surface of the bead that specifically binds to an analyte, wherein the identifier corresponds to the capture agent;
- b) a signal-emitting entity capable of directly or indirectly binding to the analyte or capture agent;
- c) at least one bead with an identifier that is recognized by the imaging processor as corresponding to a manufacturing lot;
- d) at least one bead with an identifier that is recognized by the imaging processor as corresponding to an individual;
- e) at least one bead with an identifier that is recognized by the imaging processor as corresponding to a locational identifier;
- or
- f) at least one bead that is pre-labeled for calibration of the imaging processor.
6 more elements
Dependencies are inferred from the claim text.