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US 2014/0274777 A1Published application
Lipid droplet protein markers for algal oil accumulation
AssigneeBoard of Trustees of Michigan State University
InventorsChristoph Benning, Eric R. Moellering, Chia-Hong Tsai
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Abstract
The application relates to apparatuses, methods and kits for detecting and quantifying oil content in algal samples.
Claims22
A method of quantifying oil content in an algae sample comprising quantifying Major Lipid Droplet Protein (MLDP) nucleic acid content or Major Lipid Droplet Protein (MLDP) protein content to thereby quantify oil content in the algae sample.
A method comprising:
- contacting a sample with antibodies specific for polypeptide having at least 90% amino acid sequence identity to a polypeptide having SEQ ID NO:
- 2, 4 or 6;
- incubating the test sample with the antibodies for a time sufficient and under conditions that permit antigen-antibody complex formation;
- detecting the presence of said antigen-antibody complex to thereby detect the sample's oil content.
1 more element
An apparatus or device comprising:
- a solid support comprising antibodies bound thereto;
- wherein the antibodies are specific for an polypeptide having at least 70%, amino acid sequence identity to a polypeptide having SEQ ID NO:
- 2, 4 or 6.
An apparatus or device comprising:
- a solid support comprising at least one nucleic acid probe bound thereto;
- the nucleic acid probe is specific for a mRNA having at least 70% sequence identity to a nucleic acid having SEQ ID NO:
- 1, 3, or 5.
A kit comprising:
- (a) a solid support comprising antibodies bound thereto, wherein the antibodies are specific for an polypeptide having at least 70%, amino acid sequence identity to a polypeptide having SEQ ID NO:
- 2, 4 or 6;
- (b) a solid support comprising at least one nucleic acid probe bound thereto;
- the nucleic acid probe is specific for a mRNA having at least 70% sequence identity to a nucleic acid having SEQ ID NO:
- 1, 3, or 5;
- (c) or both;
- and
- instructions for using the apparatus to detect or quantify MLDP and thereby detect or quantify oil content in a sample.
5 more elements
Dependencies are inferred from the claim text.