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US 2014/0274776 A1Published application
Porphyrin-Modified Antimicrobial Peptides for Application as Indicators of Microbial Targets
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsChris Rowe Taitt, Brandy J. White
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Abstract
Porphyrin-modified antimicrobial peptides as described here may be used as indicators of the presence of microbial targets. Their application may be as (for example) (1) fluorescent indicators in a microarray format, (2) fluorescence or absorbance based indicators in traditional solution based applications, or (3) reflectance based indicators for use in reagent-less detection platforms.
Claims15
A method of detecting, comprising:
- providing a porphyrin-modified antimicrobial peptide comprising a porphyrin covalently attached to an antimicrobial peptide;
- contacting the porphyrin-modified antimicrobial peptide with a microbial target;
- and
- detecting a change in fluorescence, absorbance, and/or reflectance of the porphyrin-modified antimicrobial peptide caused by the contacting,
- wherein the method is operable to detect the microbial targets without use of an additional optically-active reagent.
2 more elements
A method of detecting, comprising:
- providing a first porphyrin-modified antimicrobial peptide comprising a porphyrin covalently attached to a first antimicrobial peptide;
- providing a second porphyrin-modified antimicrobial peptide comprising a porphyrin covalently attached to a second antimicrobial peptide;
- contacting the first and second porphyrin-modified antimicrobial peptides with a microbial target;
- and
- detecting a change in reflectance of the first and second porphyrin-modified antimicrobial peptides caused by the contacting,
- wherein the method is operable to detect the microbial targets without use of an additional optically-active reagent.
3 more elements
A sensor comprising:
- a porphyrin-modified antimicrobial peptide comprising a porphyrin covalently attached to a antimicrobial peptide,
- wherein the sensor is operable to specifically react to a microbial target via changing fluorescence, absorbance, and/or reflectance properties of the porphyrin-modified antimicrobial peptide specifically upon contact with the microbial target.
Dependencies are inferred from the claim text.