Antibody profiling, methods and apparatus for identifying an individual or source of a biological material

A method for identifying a source of a biological material, comprising:

• contacting a sample of a biological material having individual-specific antibodies with an array including multiple proteins comprising less than about 200 proteins on a support to bind at least a portion of the individual-specific antibodies to the multiple proteins of the array, to form immune complexes;
• contacting the sample with a plurality of volume assessment spots in the array to form volume complexes, each volume assessment spot including a predetermined concentration of one or more volume determination proteins;
• applying to the array at least one detection agent comprising at least one interacting protein conjugated to a marker to detect the immune complexes and the volume complexes;
• removing non-immobilized individual-specific antibodies and unbound detection agent;
• detecting the immune complexes on the array to obtain an antibody profile;
• detecting an intensity of the volume complexes to determine if a volume of the sample is sufficient for an accurate result.

A protein array, for identifying an individual, comprising:

• a plurality of sub-arrays, each sub-array comprising:
• an array of multiple proteins comprising less than about 200 proteins immobilized on a support, wherein each protein is known, each protein is immobilized at a known predetermined location on the support, and the multiple proteins are configured to bind to at least a portion of individual-specific antibodies to form immune complexes;
• and
• a plurality of control spots as part of the array, wherein each control spot includes human Immunoglobulin G configured to form control complexes.

A protein array, for identifying an individual comprising:

• a plurality of sub-arrays, each sub-array comprising:
• an array of multiple proteins comprising less than about 200 proteins immobilized on a support, wherein each protein is known, each protein is immobilized at a known predetermined location on the support, and the multiple proteins are configured to bind to at least a portion of individual-specific antibodies to form immune complexes;
• and
• a plurality of volume assessment spots as part of the array, wherein each volume assessment spot includes a predetermined concentration of one or more volume determination proteins configured to bind to antibodies of a human to form volume complexes.