Super resolution imaging
AssigneeIllumina, Inc.
InventorsRobert C. Kain, Tarun Khurana, Kevin L. Gunderson, Yir-Shyuan Wu
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Abstract
A detection apparatus that includes (a) an array of responsive pads on a substrate surface; (b) an array of pixels, wherein each pixel in the array has a detection zone on the surface that includes a subset of at least two of the pads; and (c) an activation circuit to apply a force at a first and second pad in the subset, wherein the activation circuit is configured to apply a different force at the first pad compared to the second pad, and wherein the activation circuit has a switch to selectively alter the force at the first pad and the second pad.
Claims29
A detection apparatus, comprising
- (a) an array of responsive pads on a substrate surface,
- wherein each responsive pad comprises a nucleic acid feature of a plurality of nucleic acid features in the array,
- wherein a first subset of nucleic acid features in the plurality of nucleic acid features have a first universal sequence and different target sequences,
- wherein a second subset of nucleic acid features in the plurality of nucleic acid features have a second universal sequence and different target sequences,
- wherein the first universal sequence is different from the second universal sequence;
- (b) an array of pixels, wherein each pixel in the array has a detection zone on the surface that comprises at least two nucleic acid features of the plurality of nucleic acid features, the at least two nucleic acid features comprising a nucleic acid from the first subset of nucleic acid features and a nucleic acid from the second subset of nucleic acid features;
- and
- (c) an activation module to alter a characteristic of a pad in the first subset and of a pad in the second subset, wherein the activation module is configured to apply a different characteristic at the pad in the first subset compared to the pad in the second subset, and wherein the activation module has a switch to selectively alter the characteristic at the pads in the first and second subsets.
A method of detecting target nucleic acids, comprising
- (a) providing a substrate comprising an array of pads, the array of pads comprising a first subset of the pads and a second subset of the pads;
- (b) delivering a first solution to the substrate, wherein the first solution comprises a first plurality of different target nucleic acids that selectively attach to the first subset of pads compared to the second subset of pads;
- (c) delivering a second solution to the substrate, wherein the second solution comprises a second plurality of different target nucleic acids that selectively attach to the second subset of pads compared to the first subset of pads;
- and
- (d) detecting the substrate using an apparatus comprising an array of pixels, wherein each pixel in the array has a detection zone that comprises
- (i) at least one of the target nucleic acids that is attached to a pad of the first subset of pads, and
- (ii) at least one of the target nucleic acids that is attached to a pad of the second subset of pads.
Dependencies are inferred from the claim text.