Super resolution imaging
AssigneeIllumina, Inc.
InventorsTarun Khurana, Kevin L. Gunderson, Yir-Shyuan Wu
Life of this patent
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Abstract
A detection apparatus that includes (a) an array of responsive pads on a substrate surface; (b) an array of pixels, wherein each pixel in the array has a detection zone on the surface that includes a subset of at least two of the pads; and (c) an activation circuit to apply a force at a first and second pad in the subset, wherein the activation circuit is configured to apply a different force at the first pad compared to the second pad, and wherein the activation circuit has a switch to selectively alter the force at the first pad and the second pad.
Claims20
A detection apparatus comprising
- (a) an array of electrically responsive pads on a substrate surface;
- (b) an array of pixels, wherein each pixel in the array has a detection zone on the surface that includes a subset of at least two of the electrically responsive pads;
- and
- (c) an activation circuit to apply electric field at a first pad in the subset and a second pad in the subset, wherein the activation circuit is configured to apply different electric field at the first pad compared to the second pad, and wherein the activation circuit comprises a switch to selectively alter the electric field at the first pad compared to the second pad.
The apparatus of 2, wherein each pad is square and each detection zone includes a corner of four of the pads.
A nucleic acid sequencing system, comprising
- (a) a detection apparatus comprising
- (i) an array of electrically responsive pads on a substrate surface;
- (ii) an array of pixels, wherein each pixel in the array has a detection zone on the surface that includes a subset of four of the pads;
- and
- (iii) an activation circuit to apply an electric field to the pads in the subset individually, wherein the activation circuit is configured to apply a different electric field at a first pad of the subset compared to the other pads of the subset;
- (b) a readout circuit to acquire signals from the array of pixels;
- (c) a control module that directs the readout circuit to acquire signals from each of the pixels during a sensing period and that directs the activation circuit to sequentially apply different electric fields at the four pads during the sensing period;
- and
- (c) a processing module that correlates (i) the signals acquired from the pixels during the sensing period and (ii) the sequential application of the different electric fields at the four pads during the sensing period, in order to distinguish a sequence of signals for each of the pads.
A method of detecting analytes, comprising
- (a) providing a detection apparatus comprising an array of electrically responsive pads and an array of pixels, wherein each pixel in the array has a detection zone that includes a subset of at least two of the electrically responsive pads, wherein the two pads comprise different target analytes, respectively;
- (b) acquiring signals from each of the pixels while selectively applying an electric field at a first of the two pads to preferentially produce signal from a first of the different target analytes compared to a second of the target analytes, thereby preferentially acquiring signals from the first of the target analytes compared to the second of the target analytes;
- and
- (c) acquiring signals from each of the pixels while selectively applying an electric field at the second of the two pads to preferentially produce signal from the second of the different target analytes compared to the first of the target analytes, thereby preferentially acquiring signals from the second of the target analytes compared to the first of the target analytes.
Dependencies are inferred from the claim text.