Dna quality controls
AssigneeQiagen Sciences, LLC
InventorsYexun Wang, John DiCarlo, Vikram Devgan, Qiong Jiang, Quan Peng
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.
Claims24
A method for assessing the quality of a test genomic DNA sample, comprising:
- (a) performing one or more real-time PCR reactions that use genomic DNA in a test genomic DNA sample as templates in the presence of one or more primer pairs, wherein each of the one or more primer pairs is specific for amplifying identical or nearly identical genomic DNA fragments that are present at multiple locations in the genome of the organism from which the DNA sample is obtained,
- (b) performing one or more real-time PCR reactions that use genomic DNA in a control genomic DNA sample as templates in the presence of the one or more primer pairs used in step (a),
- (c) determining the Ct values for the one or more real-time PCR reactions in step (a), and
- (d) determining the Ct values for the one or more real-time PCR reactions in step (b),
- wherein the difference between the Ct values determined in step (c) and the corresponding Ct values determined in step (d) for the one or more real-time PCR reactions are indicative of the quality of the test genomic DNA sample.
An array for assessing the quality of a test genomic DNA sample, comprising a solid support and multiple compartments in the solid support, wherein a first primer pair specific to a first genomic DNA fragment in the test genomic DNA sample is contained in a first compartment or each of a first set of compartments, and wherein (a) the first genomic DNA fragment and (b) one or more fragments nearly identical to the first genomic DNA fragment, if present in the genome of the organism from which the DNA sample is obtained are located at multiple sites in the genome.
A kit for assessing the quality of a test genomic DNA sample, comprising:
- one or more primer pairs specific to one or more genomic DNA fragments in a test genomic DNA sample, wherein for each of the one or more genomic DNA fragments, (a) the genomic DNA fragment itself and (b) one or more fragments nearly identical to the genomic DNA fragment, if present in the genome of the organism from which the test genomic DNA sample is obtained, are located at multiple sites in the genome.
Dependencies are inferred from the claim text.