US 2014/0274707 A1 · FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS FROM PATHOGENS, AND IMMOBILIZING BACILLUS SPORES ON PLANT ROOTS

A fusion protein comprising at least one plant growth stimulating protein or peptide or at least one root binding protein or peptide and:

(a) a targeting sequence comprising an amino acid sequence having at least about 43% identity with amino acids 20-35 of SEQ ID NO:
1, wherein the identity with amino acids 25-35 is at least about 54%;
(b) a targeting sequence comprising amino acids 1-35 of SEQ ID NO:
1;
(c) a targeting sequence comprising amino acids 20-35 of SEQ ID NO:
1;
(d) a targeting sequence comprising SEQ ID NO:
1;
(e) an exosporium protein comprising SEQ ID NO:
2;
(f) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
3;
(g) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
3;
(h) a targeting sequence comprising SEQ ID NO:
3;
(i) an exosporium protein comprising SEQ ID NO:
4;
(j) a targeting sequence comprising amino acids 1-38 of SEQ ID NO:
5;
(k) a targeting sequence comprising amino acids 23-38 of SEQ ID NO:
5;
(l) a targeting sequence comprising SEQ ID NO:
5;
(m) an exosporium protein comprising SEQ ID NO:
6;
(n) a targeting sequence comprising amino acids 1-28 of SEQ ID NO:
7;
(o) a targeting sequence comprising amino acids 13-28 of SEQ ID NO:
7;
(p) a targeting sequence comprising SEQ ID NO:
7;
(q) an exosporium protein comprising SEQ ID NO:
8;
(r) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
9;
(s) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
9;
(t) a targeting sequence comprising SEQ ID NO. 9;
(u) an exosporium protein comprising SEQ ID NO. 10;
(v) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:11;
(w) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
11;
(x) a targeting sequence comprising SEQ ID NO:
11;
(y) an exosporium protein comprising SEQ ID NO:
12;
(z) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
13;
(aa) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
13;
(ab) a targeting sequence comprising SEQ ID NO:13;
(ac) an exosporium protein comprising SEQ ID NO:14;
(ad) a targeting sequence comprising amino acids 1-43 of SEQ ID NO:
15;
(ae) a targeting sequence comprising amino acids 28-43 of SEQ ID NO:
15;
(af) a targeting sequence comprising SEQ ID NO:15;
(ag) an exosporium protein comprising SEQ ID NO:16;
(ah) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
17;
(ai) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
17;
(aj) a targeting sequence comprising SEQ ID NO:17;
(ak) an exosporium protein comprising SEQ ID NO:18;
(al) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
19;
(am) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
19;
(an) a targeting sequence comprising SEQ ID NO:19;
(ao) an exosporium protein comprising SEQ ID NO:20;
(ap) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
21;
(aq) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
21;
(ar) a targeting sequence comprising SEQ ID NO:21;
(as) an exosporium protein comprising SEQ ID NO:22;
(at) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
23;
(au) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
23;
(av) a targeting sequence comprising SEQ ID NO:23;
(aw) an exosporium protein comprising SEQ ID NO:24;
(ax) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
25;
(ay) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
25;
(az) a targeting sequence comprising SEQ ID NO:25;
(ba) an exosporium protein comprising SEQ ID NO:26;
(bb) a targeting sequence comprising amino acids 1-30 of SEQ ID NO:
27;
(bc) a targeting sequence comprising amino acids 15-30 of SEQ ID NO:
27;
(bd) a targeting sequence comprising SEQ ID NO:27;
(be) an exosporium protein comprising SEQ ID NO:28;
(bf) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
29;
(bg) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
29;
(bh) a targeting sequence comprising SEQ ID NO:29;
(bi) an exosporium protein comprising SEQ ID NO:30;
(bj) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
31;
(bk) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
31;
(bl) a targeting sequence comprising SEQ ID NO:31;
(bm) an exosporium protein comprising SEQ ID NO:32;
(bn) a targeting sequence comprising amino acids 1-15 of SEQ ID NO:
33;
(bo) a targeting sequence comprising SEQ ID NO:33;
(bp) an exosporium protein comprising SEQ ID NO:34;
(bq) a targeting sequence comprising amino acids 1-16 of SEQ ID NO:
35;
(br) a targeting sequence comprising SEQ ID NO:35;
or
(bs) an exosporium protein comprising SEQ ID NO:36;
wherein the plant growth stimulating protein or peptide comprises a peptide hormone, a non-hormone peptide, or an enzyme involved in the production of a plant growth stimulating compound.

114 more elements

A fusion protein comprising at least one protein or peptide that protects a plant from a pathogen and:

(a) a targeting sequence consisting of a 16 amino acid sequence having at least about 43% identity with amino acids 20-35 of SEQ ID NO:
1, wherein the identity with amino acids 25-35 is at least about 54%;
(b) a targeting sequence consisting of amino acids 1-35 of SEQ ID NO:
1;
(c) a targeting sequence consisting of amino acids 20-35 of SEQ ID NO:
1;
(d) a targeting sequence consisting of SEQ ID NO:
1;
(e) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
3;
(f) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
3;
(g) a targeting sequence comprising SEQ ID NO:
3;
(h) an exosporium protein comprising SEQ ID NO:
4;
(i) a targeting sequence comprising amino acids 1-38 of SEQ ID NO:
5;
(j) a targeting sequence comprising amino acids 23-38 of SEQ ID NO:
5;
(k) a targeting sequence comprising SEQ ID NO:
5;
(l) an exosporium protein comprising SEQ ID NO:
6;
(m) a targeting sequence comprising amino acids 1-28 of SEQ ID NO:
7;
(n) a targeting sequence comprising amino acids 13-28 of SEQ ID NO:
7;
(o) a targeting sequence comprising SEQ ID NO:
7;
(p) an exosporium protein comprising SEQ ID NO:
8;
(q) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
9;
(r) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
9;
(s) a targeting sequence comprising SEQ ID NO. 9;
(t) an exosporium protein comprising SEQ ID NO. 10;
(u) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:11;
(v) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
11;
(w) a targeting sequence comprising SEQ ID NO:
11;
(x) an exosporium protein comprising SEQ ID NO:
12;
(y) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
13;
(z) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
13;
(aa) a targeting sequence comprising amino SEQ ID NO:13;
(ab) an exosporium protein comprising SEQ ID NO:14;
(ac) a targeting sequence comprising amino acids 1-43 of SEQ ID NO:
15;
(ad) a targeting sequence comprising amino acids 28-43 of SEQ ID NO:
15;
(ae) a targeting sequence comprising amino SEQ ID NO:15;
(af) an exosporium protein comprising SEQ ID NO:16;
(ag) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
17;
(ah) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
17;
(ai) a targeting sequence comprising amino SEQ ID NO:17;
(af) an exosporium protein comprising SEQ ID NO:18;
(ak) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
19;
(al) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
19;
(am) a targeting sequence comprising amino SEQ ID NO:19;
(an) an exosporium protein comprising SEQ ID NO:20;
(ao) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
21;
(ap) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
21;
(aq) a targeting sequence comprising amino SEQ ID NO:21;
(ar) an exosporium protein comprising SEQ ID NO:22;
(as) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
23;
(at) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
23;
(au) a targeting sequence comprising amino SEQ ID NO:23;
(av) an exosporium protein comprising SEQ ID NO:24;
(aw) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
25;
(ax) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
25;
(ay) a targeting sequence comprising amino SEQ ID NO:25;
(az) an exosporium protein comprising SEQ ID NO:26;
(ba) a targeting sequence comprising amino acids 1-30 of SEQ ID NO:
27;
(bb) a targeting sequence comprising amino acids 15-30 of SEQ ID NO:
27;
(bc) a targeting sequence comprising amino SEQ ID NO:27;
(bd) an exosporium protein comprising SEQ ID NO:28;
(be) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
29;
(bf) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
29;
(bg) a targeting sequence comprising amino SEQ ID NO:29;
(bh) an exosporium protein comprising SEQ ID NO:30;
(bi) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
31;
(bj) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
31;
(bk) a targeting sequence comprising amino SEQ ID NO:31;
(bl) an exosporium protein comprising SEQ ID NO:32;
(bm) a targeting sequence comprising amino acids 1-15 of SEQ ID NO:
33;
(bn) a targeting sequence comprising amino SEQ ID NO:33;
(bo) an exosporium protein comprising SEQ ID NO:34;
(bp) a targeting sequence comprising amino acids 1-16 of SEQ ID NO:
35;
(bq) a targeting sequence comprising amino SEQ ID NO:35;
or
(br) an exosporium protein comprising SEQ ID NO:36.

111 more elements

A fusion protein comprising at least one protein or peptide that protects a plant from a pathogen and:

(a) a targeting sequence comprising an amino acid sequence having at least about 43% identity with amino acids 20-35 of SEQ ID NO:
1, wherein the identity with amino acids 25-35 is at least about 54%;
(b) a targeting sequence comprising amino acids 1-35 of SEQ ID NO:
1;
(c) a targeting sequence comprising amino acids 20-35 of SEQ ID NO:
1;
(d) a targeting sequence comprising SEQ ID NO:
1;
(e) an exosporium protein comprising SEQ ID NO:
2;
(f) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
3;
(g) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
3;
(h) a targeting sequence comprising SEQ ID NO:
3;
(i) an exosporium protein comprising SEQ ID NO:
4;
(j) a targeting sequence comprising amino acids 1-38 of SEQ ID NO:
5;
(k) a targeting sequence comprising amino acids 23-38 of SEQ ID NO:
5;
(l) a targeting sequence comprising SEQ ID NO:
5;
(m) an exosporium protein comprising SEQ ID NO:
6;
(n) a targeting sequence comprising amino acids 1-28 of SEQ ID NO:
7;
(o) a targeting sequence comprising amino acids 13-28 of SEQ ID NO:
7;
(p) a targeting sequence comprising SEQ ID NO:
7;
(q) an exosporium protein comprising SEQ ID NO:
8;
(r) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
9;
(s) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
9;
(t) a targeting sequence comprising SEQ ID NO. 9;
(u) an exosporium protein comprising SEQ ID NO. 10;
(v) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:11;
(w) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
11;
(x) a targeting sequence comprising SEQ ID NO:
11;
(y) an exosporium protein comprising SEQ ID NO:
12;
(z) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
13;
(aa) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
13;
(ab) a targeting sequence comprising amino SEQ ID NO:13;
(ac) an exosporium protein comprising SEQ ID NO:14;
(ad) a targeting sequence comprising amino acids 1-43 of SEQ ID NO:
15;
(ae) a targeting sequence comprising amino acids 28-43 of SEQ ID NO:
15;
(af) a targeting sequence comprising amino SEQ ID NO:15;
(ag) an exosporium protein comprising SEQ ID NO:16;
(ah) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
17;
(ai) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
17;
(aj) a targeting sequence comprising amino SEQ ID NO:17;
(ak) an exosporium protein comprising SEQ ID NO:18;
(al) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
19;
(am) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
19;
(an) a targeting sequence comprising amino SEQ ID NO:19;
(ao) an exosporium protein comprising SEQ ID NO:20;
(ap) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
21;
(aq) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
21;
(ar) a targeting sequence comprising amino SEQ ID NO:21;
(as) an exosporium protein comprising SEQ ID NO:22;
(at) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
23;
(au) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
23;
(av) a targeting sequence comprising amino SEQ ID NO:23;
(aw) an exosporium protein comprising SEQ ID NO:24;
(ax) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
25;
(ay) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
25;
(az) a targeting sequence comprising amino SEQ ID NO:25;
(ba) an exosporium protein comprising SEQ ID NO:26;
(bb) a targeting sequence comprising amino acids 1-30 of SEQ ID NO:
27;
(bc) a targeting sequence comprising amino acids 15-30 of SEQ ID NO:
27;
(bd) a targeting sequence comprising amino SEQ ID NO:27;
(be) an exosporium protein comprising SEQ ID NO:28;
(bf) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
29;
(bg) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
29;
(bh) a targeting sequence comprising amino SEQ ID NO:29;
(bi) an exosporium protein comprising SEQ ID NO:30;
(bj) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
31;
(bk) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
31;
(bl) a targeting sequence comprising amino SEQ ID NO:31;
(bm) an exosporium protein comprising SEQ ID NO:32;
(bn) a targeting sequence comprising amino acids 1-15 of SEQ ID NO:
33;
(bo) a targeting sequence comprising amino SEQ ID NO:33;
(bp) an exosporium protein comprising SEQ ID NO:34;
(bq) a targeting sequence comprising amino acids 1-16 of SEQ ID NO:
35;
(br) a targeting sequence comprising amino SEQ ID NO:35;
or
(bs) an exosporium protein comprising SEQ ID NO:36;
wherein the protein or peptide that protects a plant from a pathogen comprises a harpin, an α-elastin, a β-elastin, a cryptogein, a flagellin protein, a flagellin peptide, a bacteriocin, a lysozyme, a siderophore, a conalbumin, an albumin, a lactoferrin, or a lactoferrin peptide;
or wherein the protein that protects a plant from a pathogen comprises an enzyme.

115 more elements

A method for stimulating plant growth comprising introducing a recombinant Bacillus cereus family member expressing a fusion protein into a plant growth medium or applying a recombinant Bacillus cereus family member expressing a fusion protein to foliage of a plant, a plant seed, or an area surrounding a plant, wherein the fusion protein comprises at least one plant growth stimulating protein or peptide and:

(a) a targeting sequence comprising an amino acid sequence having at least about 43% identity with amino acids 20-35 of SEQ ID NO:
1, wherein the identity with amino acids 25-35 is at least about 54%;
(b) a targeting sequence comprising amino acids 1-35 of SEQ ID NO:
1;
(c) a targeting sequence comprising amino acids 20-35 of SEQ ID NO:
1;
(d) a targeting sequence comprising SEQ ID NO:
1;
(e) an exosporium protein comprising SEQ ID NO:
2;
(f) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
3;
(g) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
3;
(h) a targeting sequence comprising SEQ ID NO:
3;
(i) an exosporium protein comprising SEQ ID NO:
4;
(j) a targeting sequence comprising amino acids 1-38 of SEQ ID NO:
5;
(k) a targeting sequence comprising amino acids 23-38 of SEQ ID NO:
5;
(l) a targeting sequence comprising SEQ ID NO:
5;
(m) an exosporium protein comprising SEQ ID NO:
6;
(n) a targeting sequence comprising amino acids 1-28 of SEQ ID NO:
7;
(o) a targeting sequence comprising amino acids 13-28 of SEQ ID NO:
7;
(p) a targeting sequence comprising SEQ ID NO:
7;
(q) an exosporium protein comprising SEQ ID NO:
8;
(r) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
9;
(s) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
9;
(t) a targeting sequence comprising SEQ ID NO. 9;
(u) an exosporium protein comprising SEQ ID NO. 10;
(v) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:11;
(w) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
11;
(x) a targeting sequence comprising SEQ ID NO:
11;
(y) an exosporium protein comprising SEQ ID NO:
12;
(z) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
13;
(aa) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
13;
(ab) a targeting sequence comprising amino SEQ ID NO:13;
(ac) an exosporium protein comprising SEQ ID NO:14;
(ad) a targeting sequence comprising amino acids 1-43 of SEQ ID NO:
15;
(ae) a targeting sequence comprising amino acids 28-43 of SEQ ID NO:
15;
(af) a targeting sequence comprising amino SEQ ID NO:15;
(ag) an exosporium protein comprising SEQ ID NO:16;
(ah) a targeting sequence comprising amino acids 1-27 of SEQ ID NO:
17;
(ai) a targeting sequence comprising amino acids 12-27 of SEQ ID NO:
17;
(aj) a targeting sequence comprising amino SEQ ID NO:17;
(ak) an exosporium protein comprising SEQ ID NO:18;
(al) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
19;
(am) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
19;
(an) a targeting sequence comprising amino SEQ ID NO:19;
(ao) an exosporium protein comprising SEQ ID NO:20;
(ap) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
21;
(aq) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
21;
(ar) a targeting sequence comprising amino SEQ ID NO:21;
(as) an exosporium protein comprising SEQ ID NO:22;
(at) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
23;
(au) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
23;
(av) a targeting sequence comprising amino SEQ ID NO:23;
(aw) an exosporium protein comprising SEQ ID NO:24;
(ax) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
25;
(ay) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
25;
(az) a targeting sequence comprising amino SEQ ID NO:25;
(ba) an exosporium protein comprising SEQ ID NO:26;
(bb) a targeting sequence comprising amino acids 1-30 of SEQ ID NO:
27;
(bc) a targeting sequence comprising amino acids 15-30 of SEQ ID NO:
27;
(bd) a targeting sequence comprising amino SEQ ID NO:27;
(be) an exosporium protein comprising SEQ ID NO:28;
(bf) a targeting sequence comprising amino acids 1-33 of SEQ ID NO:
29;
(bg) a targeting sequence comprising amino acids 18-33 of SEQ ID NO:
29;
(bh) a targeting sequence comprising amino SEQ ID NO:29;
(bi) an exosporium protein comprising SEQ ID NO:30;
(bj) a targeting sequence comprising amino acids 1-24 of SEQ ID NO:
31;
(bk) a targeting sequence comprising amino acids 9-24 of SEQ ID NO:
31;
(bl) a targeting sequence comprising amino SEQ ID NO:31;
(bm) an exosporium protein comprising SEQ ID NO:32;
(bn) a targeting sequence comprising amino acids 1-15 of SEQ ID NO:
33;
(bo) a targeting sequence comprising amino SEQ ID NO:33;
(bp) an exosporium protein comprising SEQ ID NO:34;
(bq) a targeting sequence comprising amino acids 1-16 of SEQ ID NO:
35;
(br) a targeting sequence comprising amino SEQ ID NO:35;
or
(bs) an exosporium protein comprising SEQ ID NO:36;
and wherein the plant growth stimulating protein or peptide is physically attached to the exosporium of the recombinant Bacillus family member.

114 more elements

Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing bacillus spores on plant roots

Life of this patent

Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing bacillus spores on plant roots

Life of this patent

Abstract

Claims44