Back to results
US 2014/0273272 A1Published application
Assay with internal calibration
AssigneeAbbott Laboratories
InventorsSusan GAYDA, Qiaoqiao RUAN, Joseph P. SKINNER, Sergey Y. TETIN
Life of this patent
Estimating the term…
Abstract
Provided herein are kits and methods for assays with internal calibration.
Claims23
A kit comprising:
- a tracer analyte comprising a first label, a capture analyte-binding molecule, and a detection analyte-binding molecule comprising a second label, wherein the capture analyte-binding molecule and the detection analyte-binding molecule can concurrently bind to the tracer analyte.
A method of internally calibrating an assay, comprising:
- a) contacting a capture analyte-binding molecule with a predetermined concentration of tracer analyte labeled with a first label and a predetermined concentration of detection analyte-binding molecule labeled with a second label, wherein the capture analyte-binding molecule and the detection analyte-binding molecule concurrently bind to the tracer analyte;
- b) measuring the signal intensities obtained of the first label (I10) and of the second label (I20);
- c) determining a correction factor (F) as the ratio of the intensity of the second label (I20) to the intensity of the first label (I10);
- whereby the determination of the correction factor allows for the internal calibration of the assay.
1 more element
A method of determining the concentration of analyte in one or more test samples, comprising:
- a) contacting a capture analyte-binding molecule with a predetermined concentration of tracer analyte labeled with a first label and a predetermined concentration of detection analyte-binding molecule labeled with a second label in either the absence or presence of one or more test samples that may comprise test analyte, wherein the capture analyte-binding molecule and the detection analyte-binding molecule concurrently bind to the tracer analyte and, if present, to test analyte in said one or more test samples;
- b) measuring the signal intensities obtained in the absence of said one or more test samples of the first label (I10) and the second label (I20);
- c) determining a correction factor (F) as the ratio of the intensity of the second label (I20) to the intensity of the first label (I10);
- d) measuring the signal intensities obtained in the presence of said one or more test samples of the first label (I1) and the second label (I2);
- and
- e) determining the concentration of test analyte in said one or more test samples by dividing the ratio of the intensity of the second label (I2) to the intensity of the first label (I1) by the correction factor (F) and subtracting the predetermined concentration of tracer analyte.
3 more elements
Dependencies are inferred from the claim text.