Epoxy chemistry derived materials as mixed mode chromatography media, method for their synthesis and use

A composition comprising a compound covalently bound to a substrate, said compound having a structure according to Formula (I)

• in which n is an integer selected from 0 and 1:
• X is selected from the group consisting of NRfR21R22, SRf or ORf
• wherein
• Rf is selected from the group consisting of substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl
• R21 is selected from the group consisting of H, substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl
• R22 is selected from the group consisting of a lone pair of electrons, H, substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl
• R1, R2 and R3 are selected independently from the group consisting of halogen, OR10, NR10R11, OC(O)R12, OS(O)2R12, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and a bond to said substrate
• wherein each R10 and each R11 is selected independently from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl and a bond to a silica gel substrate
• with the proviso that at least one of R1, R2 and R3 is unsubstituted C1-C3 alkyl or OH
• each R12 is selected independently from the group consisting of substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, and substituted or unsubstituted heterocycloalkyl;
• with the proviso that at least one of R1, R2 and R3 is covalently bound to said substrate;
• L1 and L2 are linker groups independently selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl;
• and
• Y is O.