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US 2014/0273236 A1Published application
Use of Clostridial Methyltransferases for Generating Novel Strains
AssigneeCoskata, Inc.
InventorAndrew Reeves
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Abstract
This invention provides isolated polynucleotides encoding DNA Type I methyltransferase and uses thereof for improving transformation efficiencies of exogenous and endogenous plasmid DNA into Clostridial hosts.
Claims17
A method of generating a bacterial recombinant cell expressing a DNA Type I methyltransferase having biological activities, comprising:
- (a) introducing a methylation vector into a bacterial host cell wherein said methylation vector expresses a DNA Type I methyltransferase polynucleotide;
- and
- (b) introducing a transforming vector into the bacterial host cell wherein said transforming vector comprises plasmid DNA that is methylated by said methylation vector.
Dependencies are inferred from the claim text.