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US 2014/0273235 A1Published application
ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS
AssigneeRegents of the University of Minnesota
InventorsDaniel F. Voytas, Paul Atkins, Nicholas J. Baltes
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Abstract
Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein.
Claims10
A method for modifying the genomic material in a plant cell, comprising:
- (a) introducing into the cell a nucleic acid comprising a crRNA and a tracrRNA, or a chimeric cr/tracrRNA hybrid, wherein the crRNA and tracrRNA, or the cr/tracrRNA hybrid, is targeted to a sequence that is endogenous to the plant cell;
- and
- (b) introducing into the cell a Cas9 endonuclease molecule that induces a double strand break at or near the sequence to which the crRNA and tracrRNA sequence is targeted, or at or near the sequence to which the cr/tracrRNA hybrid is targeted.
The method of claim 8, wherein the plant is wheat, maize, or Setaria.
The method of claim 10, wherein the plant is tomato, soybean, tobacco, potato, or Arabidopsis.
Dependencies are inferred from the claim text.